Abstract

The goal of this project is to define the proteins and DNA sequences required for the replication of adenovirus DNA. We will concentrate on: (1) continud work aimed at characterizing the adenovirus coded proteins which have been purified from cell free replication extracts. (2) Further characterization of the DNA sequences within the inverted terminal repetition of adenovirus DNA for their role in initiation and termination of DNA synthesis. (3) Identification and characterization of the host cell proteins required for adenovirus DNA replication by purification and determination of their enzyme activities. Antibodies against these proteins will be made and used to obtain genomic clones and to examine the regulation of these genes during the cell cycle. (4) Use of the purified cell replication proteins in other cell free replication systems which we will develop, such as SV40 DNA replication.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI020460-03
Application #
3130162
Study Section
Virology Study Section (VR)
Project Start
1983-07-01
Project End
1986-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Cold Spring Harbor Laboratory
Department
Type
DUNS #
065968786
City
Cold Spring Harbor
State
NY
Country
United States
Zip Code
11724

  Publications

He, Z; Wong, J M; Maniar, H S et al. (1996) Assessing the requirements for nucleotide excision repair proteins of Saccharomyces cerevisiae in an in vitro system. J Biol Chem 271:28243-9
Loo, S; Fox, C A; Rine, J et al. (1995) The origin recognition complex in silencing, cell cycle progression, and DNA replication. Mol Biol Cell 6:741-56
Rao, H; Stillman, B (1995) The origin recognition complex interacts with a bipartite DNA binding site within yeast replicators. Proc Natl Acad Sci U S A 92:2224-8
Cullmann, G; Fien, K; Kobayashi, R et al. (1995) Characterization of the five replication factor C genes of Saccharomyces cerevisiae. Mol Cell Biol 15:4661-71
Liang, C; Weinreich, M; Stillman, B (1995) ORC and Cdc6p interact and determine the frequency of initiation of DNA replication in the genome. Cell 81:667-76
Rao, H; Marahrens, Y; Stillman, B (1994) Functional conservation of multiple elements in yeast chromosomal replicators. Mol Cell Biol 14:7643-51
Marahrens, Y; Stillman, B (1994) Replicator dominance in a eukaryotic chromosome. EMBO J 13:3395-400
Bell, S P; Marahrens, Y; Rao, H et al. (1993) The replicon model and eukaryotic chromosomes. Cold Spring Harb Symp Quant Biol 58:435-42
Bell, S P; Kobayashi, R; Stillman, B (1993) Yeast origin recognition complex functions in transcription silencing and DNA replication. Science 262:1844-9
Diffley, J F; Stillman, B (1992) DNA binding properties of an HMG1-related protein from yeast mitochondria. J Biol Chem 267:3368-74

Showing the most recent 10 out of 26 publications