The ultimate goal of our studies is to understand in molecular terms how bacteria cause diseases. A bacterial infection can be regarded as a war between the microbe and the host where the bacteria's attempt to adhere to and colonize the host tissue represent the first battle in the campaign. For our model organism, Staphylococcus aureus tissue adherence is mediated by a sub-family of bacterial surface adhesins called MSCRAMMs. In previous work, we discovered the MSCRAMMs, cloned and sequenced several MSCRAMM genes and began characterizing the encoded proteins and their interactions with host components. These studies revealed amazingly sophistical mechanisms of host tissue adherence designed to avoid inactivation by host defense systems. We hypothesize that the MSCRAMMs are in the first line of bacterial attachment and their molecular design makes them uniquely suited for this role. Consequently, we now propose a detailed molecular analysis of Staphylococcal surface proteins and their interactions with host components.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI020624-19
Application #
6510316
Study Section
Pathobiochemistry Study Section (PBC)
Project Start
1989-12-01
Project End
2005-03-31
Budget Start
2002-04-01
Budget End
2003-03-31
Support Year
19
Fiscal Year
2002
Total Cost
$425,524
Indirect Cost
Name
Texas A&M University
Department
Type
Schools of Medicine
DUNS #
City
College Station
State
TX
Country
United States
Zip Code
77845
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Galloway-Peña, Jessica R; Liang, Xiaowen; Singh, Kavindra V et al. (2015) The identification and functional characterization of WxL proteins from Enterococcus faecium reveal surface proteins involved in extracellular matrix interactions. J Bacteriol 197:882-92
Zhi, Hui; Weening, Eric H; Barbu, Elena Magda et al. (2015) The BBA33 lipoprotein binds collagen and impacts Borrelia burgdorferi pathogenesis. Mol Microbiol 96:68-83

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