Abstract

This project intends to develo an AIDS transmission blocking vaccine based on our previous finding that maternal high affinity antibodies to the gp120 MN Primary Neutralizing Domain (PND) can protect the fetus from infection by the HIV infected mother. We will first use various gp120 MN-PND peptide-conjugates in animals to determine their immunogenicity with regard to the raising of high affinity neutralizing antibodies to MN-PND. Four immunogenic carriers will be used with the peptides to include: Tetanus toxoid, Diphtheria toxoid, PPD and BCG cell walls. In addition, human volunteers will be screened, recruited and ultimately vaccinated with MN-PND Vaccine GMP conjugates. The following groups of vollunteers will be recruited: 1. A small number of normal human volunteers to test the immunogenicity of the peptide vaccine conjugates. 2. HIV positive healthy volunteers with intact humoral immunity. 3. HIV sropositive women with the probability of becoming pregnant within 1 year of vaccination. 4. Finally, after review of te results in the above groups, a randomized controlled trial in HIV seropositive pregnant women will be designed. All vaccine trials will be coordinated with the NIH Vaccine Task Force.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI020671-09
Application #
3790986
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
9
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Type
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Goldstein, H; Pettoello-Mantovani, M; Katopodis, N F et al. (1996) SCID-hu mice: a model for studying disseminated HIV infection. Semin Immunol 8:223-31
Kollmann, T R; Pettoello-Mantovani, M; Katopodis, N F et al. (1996) Inhibition of acute in vivo human immunodeficiency virus infection by human interleukin 10 treatment of SCID mice implanted with human fetal thymus and liver. Proc Natl Acad Sci U S A 93:3126-31
Kollmann, T R; Kim, A; Pettoello-Mantovani, M et al. (1995) Divergent effects of chronic HIV-1 infection on human thymocyte maturation in SCID-hu mice. J Immunol 154:907-21
Kollmann, T R; Pettoello-Mantovani, M; Zhuang, X et al. (1994) Disseminated human immunodeficiency virus 1 (HIV-1) infection in SCID-hu mice after peripheral inoculation with HIV-1. J Exp Med 179:513-22
Kollmann, T R; Kim, A; Zhuang, X et al. (1994) Reconstitution of SCID mice with human lymphoid and myeloid cells after transplantation with human fetal bone marrow without the requirement for exogenous human cytokines. Proc Natl Acad Sci U S A 91:8032-6
Rubinstein, A; Goldstein, H; Pettoello-Mantovani, M et al. (1994) Preliminary results of V3 loop peptide-primary neutralizing domain conjugate phase 1 vaccine trail. AIDS Res Hum Retroviruses 10 Suppl 2:S149-53
Pettoello-Mantovani, M; Casadevall, A; Smarnworawong, P et al. (1994) Enhancement of HIV type 1 infectivity in vitro by capsular polysaccharide of Cryptococcus neoformans and Haemophilus influenzae. AIDS Res Hum Retroviruses 10:1079-87
Kollmann, T R; Goldstein, M M; Goldstein, H (1993) The concurrent maturation of mouse and human thymocytes in human fetal thymus implanted in NIH-beige-nude-xid mice is associated with the reconstitution of the murine immune system. J Exp Med 177:821-32
Rubinstein, A; Goldstein, H; Calvelli, T et al. (1993) Maternofetal transmission of human immunodeficiency virus-1: the role of antibodies to the V3 primary neutralizing domain. Pediatr Res 33:S76-8;discussion S78-9
Suarez Jr, M A; Blanco, B; Brion, L P et al. (1993) A rapid test for the detection of human immunodeficiency virus antibodies in cord blood. J Pediatr 123:259-61

Showing the most recent 10 out of 47 publications