Maternal-Fetal Transmission of HIV

Rubinstein, Arye

Abstract

The goal of this project is to identify maternal humoral immune responses that may prevent materno fetal HIV transmission. Our studies will initially focus on maternal antibodies to the Principal Neutralizing Domain (PND) of the gp120 envelope glycoprotein of the HTLV-III MN strain. We will, however, also investigate other neutralizing epitopes to gp120-MN-PND will be measured in the sera of materno-fetal pairs, in amniotic fluid and in body secretion (secretory IgA). IgG subclass antibodies to gp120-MN-PND will also be evaluated in maternal blood, amniotic fluid and cord bloods. Purified high affinity antibodies will be assessed in vitro for their virus neutralization capacity. The presence of these antibodies will also be correlated with maternal virus load and with fetal outcome.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Research Project (R01)
• Project #: 5R01AI020671-10
• Application #: 3768967
• Support Year: 10
• Fiscal Year: 1993

Institution

• Name: Albert Einstein College of Medicine
• DUNS #: 009095365
• City: Bronx
• State: NY
• Country: United States
• Zip Code: 10461

Publications

Goldstein, H; Pettoello-Mantovani, M; Katopodis, N F et al. (1996) SCID-hu mice: a model for studying disseminated HIV infection. Semin Immunol 8:223-31

Kollmann, T R; Pettoello-Mantovani, M; Katopodis, N F et al. (1996) Inhibition of acute in vivo human immunodeficiency virus infection by human interleukin 10 treatment of SCID mice implanted with human fetal thymus and liver. Proc Natl Acad Sci U S A 93:3126-31

Kollmann, T R; Kim, A; Pettoello-Mantovani, M et al. (1995) Divergent effects of chronic HIV-1 infection on human thymocyte maturation in SCID-hu mice. J Immunol 154:907-21

Kollmann, T R; Pettoello-Mantovani, M; Zhuang, X et al. (1994) Disseminated human immunodeficiency virus 1 (HIV-1) infection in SCID-hu mice after peripheral inoculation with HIV-1. J Exp Med 179:513-22

Kollmann, T R; Kim, A; Zhuang, X et al. (1994) Reconstitution of SCID mice with human lymphoid and myeloid cells after transplantation with human fetal bone marrow without the requirement for exogenous human cytokines. Proc Natl Acad Sci U S A 91:8032-6

Rubinstein, A; Goldstein, H; Pettoello-Mantovani, M et al. (1994) Preliminary results of V3 loop peptide-primary neutralizing domain conjugate phase 1 vaccine trail. AIDS Res Hum Retroviruses 10 Suppl 2:S149-53

Pettoello-Mantovani, M; Casadevall, A; Smarnworawong, P et al. (1994) Enhancement of HIV type 1 infectivity in vitro by capsular polysaccharide of Cryptococcus neoformans and Haemophilus influenzae. AIDS Res Hum Retroviruses 10:1079-87

Kollmann, T R; Goldstein, M M; Goldstein, H (1993) The concurrent maturation of mouse and human thymocytes in human fetal thymus implanted in NIH-beige-nude-xid mice is associated with the reconstitution of the murine immune system. J Exp Med 177:821-32

Rubinstein, A; Goldstein, H; Calvelli, T et al. (1993) Maternofetal transmission of human immunodeficiency virus-1: the role of antibodies to the V3 primary neutralizing domain. Pediatr Res 33:S76-8;discussion S78-9

Suarez Jr, M A; Blanco, B; Brion, L P et al. (1993) A rapid test for the detection of human immunodeficiency virus antibodies in cord blood. J Pediatr 123:259-61