Abstract

The vaccine core laboratory will interact with pharmaceutical companies to manufacture a variety of peptide AIDS Vaccines starting with the gp120-MN-PND vaccine. In the first two years it will provide the necessary funds for the manufacturing and conjugation of peptide vaccines as these are being developed by investigators in Project II. Preliminary experimental studies in mice will be conducted to determin toxicities and immunogenicity of candidate vaccines. As the vaccine trials proceed through the investigations delineated in Projet I and II, research methods developed to monitor vaccine outcome will be adapted in the core facility for routine monitoring of vaccine outcome. Targetted antibodies will be developed in conjugation with Stratacyte Corp. (LaJolla, Ca), where all recombinant DNA work involving human monoclonal antibody expression in the ImmunoZap bacteriophage lambda expression vector, will be carried out.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI020671-10
Application #
3768970
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Type
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Goldstein, H; Pettoello-Mantovani, M; Katopodis, N F et al. (1996) SCID-hu mice: a model for studying disseminated HIV infection. Semin Immunol 8:223-31
Kollmann, T R; Pettoello-Mantovani, M; Katopodis, N F et al. (1996) Inhibition of acute in vivo human immunodeficiency virus infection by human interleukin 10 treatment of SCID mice implanted with human fetal thymus and liver. Proc Natl Acad Sci U S A 93:3126-31
Kollmann, T R; Kim, A; Pettoello-Mantovani, M et al. (1995) Divergent effects of chronic HIV-1 infection on human thymocyte maturation in SCID-hu mice. J Immunol 154:907-21
Kollmann, T R; Pettoello-Mantovani, M; Zhuang, X et al. (1994) Disseminated human immunodeficiency virus 1 (HIV-1) infection in SCID-hu mice after peripheral inoculation with HIV-1. J Exp Med 179:513-22
Kollmann, T R; Kim, A; Zhuang, X et al. (1994) Reconstitution of SCID mice with human lymphoid and myeloid cells after transplantation with human fetal bone marrow without the requirement for exogenous human cytokines. Proc Natl Acad Sci U S A 91:8032-6
Rubinstein, A; Goldstein, H; Pettoello-Mantovani, M et al. (1994) Preliminary results of V3 loop peptide-primary neutralizing domain conjugate phase 1 vaccine trail. AIDS Res Hum Retroviruses 10 Suppl 2:S149-53
Pettoello-Mantovani, M; Casadevall, A; Smarnworawong, P et al. (1994) Enhancement of HIV type 1 infectivity in vitro by capsular polysaccharide of Cryptococcus neoformans and Haemophilus influenzae. AIDS Res Hum Retroviruses 10:1079-87
Kollmann, T R; Goldstein, M M; Goldstein, H (1993) The concurrent maturation of mouse and human thymocytes in human fetal thymus implanted in NIH-beige-nude-xid mice is associated with the reconstitution of the murine immune system. J Exp Med 177:821-32
Rubinstein, A; Goldstein, H; Calvelli, T et al. (1993) Maternofetal transmission of human immunodeficiency virus-1: the role of antibodies to the V3 primary neutralizing domain. Pediatr Res 33:S76-8;discussion S78-9
Suarez Jr, M A; Blanco, B; Brion, L P et al. (1993) A rapid test for the detection of human immunodeficiency virus antibodies in cord blood. J Pediatr 123:259-61

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