: Since the inception of this project, our global hypothesis has been that microbial community dynamics in larval mosquito habitats govern larval growth and consequent adult emergence, and through direct and indirect influences of larval nutrition affect mosquito population dynamics and vectorial capacity. By understanding the controlling factors imposed by the microbial community in support or suppression of larval growth, new insights into mosquito ecology and vector control methods will be elucidated, including means of manipulating the microbial community with genetic transformation methods. In this competing continuation proposal, we will build directly upon our recent research advances with newly-framed hypotheses and new directions that involve 3 inter-related Specific Aims covering the following objectives: (1.1) Development of an axenic (germ-free) experimental system for direct analysis of the effects of individual microbial components to larval growth responses, using stable isotope tracers in field experiments and radio-isotope tracers in laboratory experiments; (1.2) Analysis of the contribution of extracellular polysaccharides associated with microbial biofilms, as well as dissolved and colloidal organic compounds, to larval nutrition and growth; (1 .3) Estimation of assimilation efficiencies of natural microbial foods; (2.1) Molecular-based characterizations of the biofilm-associated microbial community using 16S rDNA sequence libraries, and sole-carbon source utilization phenotype analysis; (2.2) Characterization of the fungat and protozoan community and biomass associated with substrate biofllms; (2.3) Analysis of the role of phosphorous limitation to microbial biomass production and mosquito growth; (3.1) Isolation on selective media of dominant Flavobacterium, Comamonas, and Pseudomonas spinosa strains; (3.2) Transformation of these bacterial strains with Cryl 1A larvicidal toxin genes by chromosomal integration using mini-TN5 transposons; and (3.3) Studies of transformant efficacy against mosquito larvae, and persistence in simulated mosquito habitats.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Project (R01)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-TMP (01))
Program Officer
Costero, Adriana
Project Start
Project End
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Fiscal Year
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Michigan State University
Schools of Arts and Sciences
East Lansing
United States
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Walker, Edward D (2016) Toxicity of Sulfide and Ammonium to Aedes triseriatus Larvae (Diptera: Culicidae) in Water-Filled Tree Holes and Tires. J Med Entomol 53:577-583
Kaufman, Michael G; Fonseca, Dina M (2014) Invasion biology of Aedes japonicus japonicus (Diptera: Culicidae). Annu Rev Entomol 59:31-49
Kaufman, Michael G; Stanuszek, William W; Brouhard, Elizabeth A et al. (2012) Establishment of Aedes japonicus japonicus and its colonization of container habitats in Michigan. J Med Entomol 49:1307-17
Hamer, Gabriel L; Donovan, Danielle J; Hood-Nowotny, Rebecca et al. (2012) Evaluation of a stable isotope method to mark naturally-breeding larval mosquitoes for adult dispersal studies. J Med Entomol 49:61-70
Pelz-Stelinski, Kirsten; Kaufman, Michael G; Walker, Edward D (2011) Beetle (Coleoptera: Scirtidae) facilitation of larval mosquito growth in tree hole habitats is linked to multitrophic microbial interactions. Microb Ecol 62:690-703
Pelz-Stelinski, K S; Walker, E D; Kaufman, M G (2010) Senescent leaf exudate increases mosquito survival and microbial activity. Ecol Entomol 35:329-340
Chen, Shicheng; Kaufman, Michael G; Bagdasarian, Michael et al. (2010) Development of an efficient expression system for Flavobacterium strains. Gene 458:1-10
Walker, E D; Kaufman, M G; Merritt, R W (2010) An acute trophic cascade among microorganisms in the tree hole ecosystem following removal of omnivorous mosquito larvae. Community Ecol 11:171-178
Kaufman, Michael G; Pelz-Stelinski, Kirsten S; Yee, Donald A et al. (2010) Stable Isotope Analysis Reveals Detrital Resource Base Sources of the Tree Hole Mosquito, Aedes triseriatus. Ecol Entomol 35:586-593
Loss, Scott R; Hamer, Gabriel L; Goldberg, Tony L et al. (2009) Nestling passerines are not important hosts for amplification of West Nile Virus in Chicago, Illinois. Vector Borne Zoonotic Dis 9:13-8

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