Abstract

Type 1 pili of E. coli mediate adhesion to a variety of eukaryotic cells and the investigator has collaborated in studies that show that their major role in """"""""virulence"""""""" is to promote communicability of E. coli from animal to animal. The PI's research focuses on the way that type 1 pili interact with eucaryotic cells and how certain minor pilus components influence pilus biogenesis. The intent is to enable intervention in colonization and spread of pathogenic bacteria and to learn more about mechanisms of assembly of polymerized supramolecular structures in general.
Specific aims are 1. Examine the molecular interactions in receptor binding and 2. examine molecular interactions in pilus assembly.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI022223-13
Application #
2886482
Study Section
Bacteriology and Mycology Subcommittee 2 (BM)
Program Officer
Heyse, Stephen P
Project Start
1985-04-01
Project End
2001-07-31
Budget Start
1999-08-01
Budget End
2001-07-31
Support Year
13
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
North Carolina State University Raleigh
Department
Microbiology/Immun/Virology
Type
Schools of Veterinary Medicine
DUNS #
City
Raleigh
State
NC
Country
United States
Zip Code
27695

  Publications

Bollinger, R Randal; Everett, Mary Lou; Wahl, Shaina D et al. (2006) Secretory IgA and mucin-mediated biofilm formation by environmental strains of Escherichia coli: role of type 1 pili. Mol Immunol 43:378-87
Orndorff, Paul E; Devapali, Aditya; Palestrant, Sarah et al. (2004) Immunoglobulin-mediated agglutination of and biofilm formation by Escherichia coli K-12 require the type 1 pilus fiber. Infect Immun 72:1929-38
Hamrick, Terri S; Diaz, Adam H; Havell, Edward A et al. (2003) Influence of extracellular bactericidal agents on bacteria within macrophages. Infect Immun 71:1016-9
Valenski, Mary L; Harris, Sandra L; Spears, Patricia A et al. (2003) The Product of the fimI gene is necessary for Escherichia coli type 1 pilus biosynthesis. J Bacteriol 185:5007-11
Harris, S L; Spears, P A; Havell, E A et al. (2001) Characterization of Escherichia coli type 1 pilus mutants with altered binding specificities. J Bacteriol 183:4099-102
Woodall, L D; Russell, P W; Harris, S L et al. (1993) Rapid, synchronous, and stable induction of type 1 piliation in Escherichia coli by using a chromosomal lacUV5 promoter. J Bacteriol 175:2770-8
Scott, J R; Wakefield, J C; Russell, P W et al. (1992) CooB is required for assembly but not transport of CS1 pilin. Mol Microbiol 6:293-300
Russell, P W; Orndorff, P E (1992) Lesions in two Escherichia coli type 1 pilus genes alter pilus number and length without affecting receptor binding. J Bacteriol 174:5923-35
Kawula, T H; Orndorff, P E (1991) Rapid site-specific DNA inversion in Escherichia coli mutants lacking the histonelike protein H-NS. J Bacteriol 173:4116-23
Bloch, C A; Orndorff, P E (1990) Impaired colonization by and full invasiveness of Escherichia coli K1 bearing a site-directed mutation in the type 1 pilin gene. Infect Immun 58:275-8

Showing the most recent 10 out of 16 publications