Abstract

The proposed program will continue our studies on the mechanisms involved in the growth of picornaviruses. The major focus will be on poliovirus RNA replication. Purification of both the host and viral proteins that make up the replicase system is planned. The mechanism of initiation of viral RNA replication and the possible involvement of a protein primer will be examined. Three other areas of proposed investigation are: the mechanism of virus-induced inhibition of host cell protein synthesis; studies of the role of protein NCVPX in the viral life cycle; and derivation of a cloned DNA replica of the poliovirus genome in an E. coli plasmid and studies of its infectivity and structure.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI022346-02
Application #
3133307
Study Section
Virology Study Section (VR)
Project Start
1984-07-01
Project End
1985-12-31
Budget Start
1985-01-01
Budget End
1985-12-31
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Whitehead Institute for Biomedical Research
Department
Type
DUNS #
076580745
City
Cambridge
State
MA
Country
United States
Zip Code

  Publications

Connor, R I; Chen, B K; Choe, S et al. (1995) Vpr is required for efficient replication of human immunodeficiency virus type-1 in mononuclear phagocytes. Virology 206:935-44
Gurish, M F; Pear, W S; Stevens, R L et al. (1995) Tissue-regulated differentiation and maturation of a v-abl-immortalized mast cell-committed progenitor. Immunity 3:175-86
Saksela, K; Cheng, G; Baltimore, D (1995) Proline-rich (PxxP) motifs in HIV-1 Nef bind to SH3 domains of a subset of Src kinases and are required for the enhanced growth of Nef+ viruses but not for down-regulation of CD4. EMBO J 14:484-91
Saksela, K; Stevens, C; Rubinstein, P et al. (1994) Human immunodeficiency virus type 1 mRNA expression in peripheral blood cells predicts disease progression independently of the numbers of CD4+ lymphocytes. Proc Natl Acad Sci U S A 91:1104-8
Chen, B K; Saksela, K; Andino, R et al. (1994) Distinct modes of human immunodeficiency virus type 1 proviral latency revealed by superinfection of nonproductively infected cell lines with recombinant luciferase-encoding viruses. J Virol 68:654-60
Andino, R; Silvera, D; Suggett, S D et al. (1994) Engineering poliovirus as a vaccine vector for the expression of diverse antigens. Science 265:1448-51
Cheng, G; Ye, Z S; Baltimore, D (1994) Binding of Bruton's tyrosine kinase to Fyn, Lyn, or Hck through a Src homology 3 domain-mediated interaction. Proc Natl Acad Sci U S A 91:8152-5
Saksela, K; Muchmore, E; Girard, M et al. (1993) High viral load in lymph nodes and latent human immunodeficiency virus (HIV) in peripheral blood cells of HIV-1-infected chimpanzees. J Virol 67:7423-7
Andino, R; Rieckhof, G E; Achacoso, P L et al. (1993) Poliovirus RNA synthesis utilizes an RNP complex formed around the 5'-end of viral RNA. EMBO J 12:3587-98
Li, J P; Baltimore, D (1991) Mechanism of leukemogenesis induced by mink cell focus-forming murine leukemia viruses. J Virol 65:2408-14

Showing the most recent 10 out of 24 publications