Equine infectious anemia virus (EIAV) is unique among lentivirus systems in that infected horses routinely progress from chronic disease characterized by recurring viremias and clinical episodes to a long term inapparent state in which host immune responses maintain a strict control of virus replication and prevent clinical signs of disease. Thus EIAV serves as a novel natural model for the immunologic control of a lentivirus infection, despite the array of persistence and escape mechanisms employed by EIAV. The identification of the specific humoral and cellular immune responses that evolve to overcome aggressive EIAV replication and disease can provide important insights into strategies for the development of immunization procedures for the prevention or treatment of HIV infection and disease in humans. The current competitive renewal application proposes the following specific aims: (1) To extend the analysis of linear and conformational B- and T-cell determinants of the major antigens of EIAV by using an extensive panel of recombinant and synthetic peptide antigens to analyze the targets of humoral and cellular immune responses in inapparently infected ponies; (2) To characterize the evolution of EIAV-specific immune responses as experimentally infected ponies progress from chronic EIA to the inapparent State. Using the panel of antigens and assays defined in the preceding specific aim, this longitudinal study will determine the kinetics of immune responses with respect to the progression of EIAV infection and disease; and (3) To evaluate selected immunogens and vaccination strategies for their ability to elicit protective immune responses against EIAV infection and disease.
This specific aim will be a direct testing of the immune correlates indicated in the preceding two specific aims to determine if experimental immunization strategies can elicit immune protection comparable to the immune status achieved in persistent EIAV infections.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI025850-08
Application #
2063117
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Project Start
1987-09-30
Project End
1997-04-30
Budget Start
1994-05-01
Budget End
1995-04-30
Support Year
8
Fiscal Year
1994
Total Cost
Indirect Cost
Name
University of Pittsburgh
Department
Genetics
Type
Schools of Medicine
DUNS #
053785812
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Craigo, Jodi K; Ezzelarab, Corin; Cook, Sheila J et al. (2015) Protective efficacy of centralized and polyvalent envelope immunogens in an attenuated equine lentivirus vaccine. PLoS Pathog 11:e1004610
Liu, Chong; Cook, Sheila J; Craigo, Jodi K et al. (2014) Epitope shifting of gp90-specific cellular immune responses in EIAV-infected ponies. Vet Immunol Immunopathol 161:161-9
Craigo, Jodi K; Montelaro, Ronald C (2013) Lessons in AIDS vaccine development learned from studies of equine infectious, anemia virus infection and immunity. Viruses 5:2963-76
Craigo, Jodi K; Ezzelarab, Corin; Cook, Sheila J et al. (2013) Envelope determinants of equine lentiviral vaccine protection. PLoS One 8:e66093
Craigo, Jodi K; Ezzelarab, Corin; Montelaro, Ronald C (2012) Development of a high throughput, semi-automated, infectious center cell-based ELISA for equine infectious anemia virus. J Virol Methods 185:221-7
Liu, Chong; Cook, Frank R; Cook, Sheila J et al. (2012) The determination of in vivo envelope-specific cell-mediated immune responses in equine infectious anemia virus-infected ponies. Vet Immunol Immunopathol 148:302-10
Craigo, Jodi K; Montelaro, Ronald C (2011) EQUINE INFECTIOUS ANEMIA VIRUS INFECTION AND IMMUNITY: LESSONS FOR AIDS VACCINE DEVELOPMENT. Future Virol 6:139-142
Craigo, Jodi K; Montelaro, Ronald C (2010) EIAV envelope diversity: shaping viral persistence and encumbering vaccine efficacy. Curr HIV Res 8:81-6
Craigo, Jodi K; Barnes, Shannon; Cook, Sheila J et al. (2010) Divergence, not diversity of an attenuated equine lentivirus vaccine strain correlates with protection from disease. Vaccine 28:8095-104
Fidalgo-Carvalho, Isabel; Craigo, Jodi K; Barnes, Shannon et al. (2009) Characterization of an equine macrophage cell line: application to studies of EIAV infection. Vet Microbiol 136:8-19

Showing the most recent 10 out of 64 publications