investigator=s abstract): The goal of this proposal is to elucidate the role of Gag proteins in virion assembly, virus-induced cytopathicity, virus host -range and early steps of virus transmission. Preliminary studies indicate the Gag polyprotein, and in particular its matrix domain, has a central role in controlling HIV-1 induced cytopathicity. Furthermore, data show that matrix, and its interaction partner, the cytoplasmic domain of the viral envelope glycoprotein, can have major effects on the target cell tropism of HIV-1. To elucidate the basis for this novel role of Gag, which may have important implications for HIV-1 pathogenicity, the investigator proposes to investigate whether there is a connection between the role of Gag in viral cytopathicity and viral host range. In addition, the investigator has developed a method for efficient purification of intact HIV-1 virion cores and proposes to examine their protein composition and structure.
| Popov, Sergei; Popova, Elena; Inoue, Michio et al. (2018) HIV-1 gag recruits PACSIN2 to promote virus spreading. Proc Natl Acad Sci U S A 115:7093-7098 |
| Usami, Yoshiko; Wu, Yuanfei; Göttlinger, Heinrich G (2015) SERINC3 and SERINC5 restrict HIV-1 infectivity and are counteracted by Nef. Nature 526:218-23 |
| Caillat, Christophe; Macheboeuf, Pauline; Wu, Yuanfei et al. (2015) Asymmetric ring structure of Vps4 required for ESCRT-III disassembly. Nat Commun 6:8781 |
| Martinelli, Nicolas; Hartlieb, Bettina; Usami, Yoshiko et al. (2012) CC2D1A is a regulator of ESCRT-III CHMP4B. J Mol Biol 419:75-88 |
| Lata, Suman; Schoehn, Guy; Solomons, Julianna et al. (2009) Structure and function of ESCRT-III. Biochem Soc Trans 37:156-60 |
| Usami, Yoshiko; Popov, Sergei; Popova, Elena et al. (2009) The ESCRT pathway and HIV-1 budding. Biochem Soc Trans 37:181-4 |
| Calistri, Arianna; Del Vecchio, Claudia; Salata, Cristiano et al. (2009) Role of the feline immunodeficiency virus L-domain in the presence or absence of Gag processing: involvement of ubiquitin and Nedd4-2s ligase in viral egress. J Cell Physiol 218:175-82 |
| Pizzato, Massimo; Popova, Elena; Gottlinger, Heinrich G (2008) Nef can enhance the infectivity of receptor-pseudotyped human immunodeficiency virus type 1 particles. J Virol 82:10811-9 |
| Ma, Yu May; Boucrot, Emmanuel; Villen, Judit et al. (2007) Targeting of AMSH to endosomes is required for epidermal growth factor receptor degradation. J Biol Chem 282:9805-12 |
| Calistri, Arianna; Sette, Paola; Salata, Cristiano et al. (2007) Intracellular trafficking and maturation of herpes simplex virus type 1 gB and virus egress require functional biogenesis of multivesicular bodies. J Virol 81:11468-78 |
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