Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI032957-12
Application #
6761856
Study Section
Virology Study Section (VR)
Program Officer
Dempsey, Walla L
Project Start
1992-07-01
Project End
2007-12-31
Budget Start
2004-01-01
Budget End
2004-12-31
Support Year
12
Fiscal Year
2004
Total Cost
$286,125
Indirect Cost
Name
Johns Hopkins University
Department
Pharmacology
Type
Schools of Medicine
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Gibson, W (2008) Structure and formation of the cytomegalovirus virion. Curr Top Microbiol Immunol 325:187-204
Nguyen, Nang L; Loveland, Amy N; Gibson, Wade (2008) Nuclear localization sequences in cytomegalovirus capsid assembly proteins (UL80 proteins) are required for virus production: inactivating NLS1, NLS2, or both affects replication to strikingly different extents. J Virol 82:5381-9
Brignole, Edward J; Gibson, Wade (2007) Enzymatic activities of human cytomegalovirus maturational protease assemblin and its precursor (pPR, pUL80a) are comparable: [corrected] maximal activity of pPR requires self-interaction through its scaffolding domain. J Virol 81:4091-103
Loveland, Amy N; Nguyen, Nang L; Brignole, Edward J et al. (2007) The amino-conserved domain of human cytomegalovirus UL80a proteins is required for key interactions during early stages of capsid formation and virus production. J Virol 81:620-8
McCartney, Stephen A; Brignole, Edward J; Kolegraff, Keli N et al. (2005) Chemical rescue of I-site cleavage in living cells and in vitro discriminates between the cytomegalovirus protease, assemblin, and its precursor, pUL80a. J Biol Chem 280:33206-12
Loveland, Amy N; Chan, Chee-Kai; Brignole, Edward J et al. (2005) Cleavage of human cytomegalovirus protease pUL80a at internal and cryptic sites is not essential but enhances infectivity. J Virol 79:12961-8
Casaday, Rebecca J; Bailey, Justin R; Kalb, Suzanne R et al. (2004) Assembly protein precursor (pUL80.5 homolog) of simian cytomegalovirus is phosphorylated at a glycogen synthase kinase 3 site and its downstream ""priming"" site: phosphorylation affects interactions of protein with itself and with major capsid protein. J Virol 78:13501-11
Chan, Chee-Kai; Brignole, Edward J; Gibson, Wade (2002) Cytomegalovirus assemblin (pUL80a): cleavage at internal site not essential for virus growth; proteinase absent from virions. J Virol 76:8667-74
Plafker, S M; Gibson, W (1998) Cytomegalovirus assembly protein precursor and proteinase precursor contain two nuclear localization signals that mediate their own nuclear translocation and that of the major capsid protein. J Virol 72:7722-32
Gibson, W; Hall, M R (1997) Assemblin, an essential herpesvirus proteinase. Drug Des Discov 15:39-47

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