Abstract

The parasite Toxoplasma gondii is both an important human pathogen and a model for other related intracellular parasites that are grouped in the phylum Apicomplexa. Intracellular survival requires a large number of adaptations many of which are mediated by regulated secretion of parasite proteins. Toxoplasma resides within an intracellular vacuole that is endowed with the ability to resist intracellular digestion and to support parasite growth. Like related apicomplexan parasites, Toxoplasma is highly specialized for proteins secretion having three distinct compartments that discharge sequentially on invasion of the host cell. They will define the mechanisms of calcium-regulated secretion in this organism and investigate the role of secretory proteins intracellular survival.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI034036-10
Application #
6510651
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Program Officer
Wali, Tonu M
Project Start
1993-04-01
Project End
2005-03-31
Budget Start
2002-04-01
Budget End
2003-03-31
Support Year
10
Fiscal Year
2002
Total Cost
$308,000
Indirect Cost

  Institution

Name
Washington University
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Long, Shaojun; Brown, Kevin M; Sibley, L David (2018) CRISPR-mediated Tagging with BirA Allows Proximity Labeling in Toxoplasma gondii. Bio Protoc 8:
Brown, Kevin M; Sibley, L David (2018) Essential cGMP Signaling in Toxoplasma Is Initiated by a Hybrid P-Type ATPase-Guanylate Cyclase. Cell Host Microbe 24:804-816.e6
Brown, Kevin M; Long, Shaojun; Sibley, L David (2018) Conditional Knockdown of Proteins Using Auxin-inducible Degron (AID) Fusions in Toxoplasma gondii. Bio Protoc 8:
Shen, Bang; Brown, Kevin; Long, Shaojun et al. (2017) Development of CRISPR/Cas9 for Efficient Genome Editing in Toxoplasma gondii. Methods Mol Biol 1498:79-103
Jones, Nathaniel G; Wang, Qiuling; Sibley, L David (2017) Secreted protein kinases regulate cyst burden during chronic toxoplasmosis. Cell Microbiol 19:
Long, Shaojun; Anthony, Bryan; Drewry, Lisa L et al. (2017) A conserved ankyrin repeat-containing protein regulates conoid stability, motility and cell invasion in Toxoplasma gondii. Nat Commun 8:2236
Garcia, Celia R S; Alves, Eduardo; Pereira, Pedro H S et al. (2017) InsP3 Signaling in Apicomplexan Parasites. Curr Top Med Chem 17:2158-2165
Long, Shaojun; Brown, Kevin M; Drewry, Lisa L et al. (2017) Calmodulin-like proteins localized to the conoid regulate motility and cell invasion by Toxoplasma gondii. PLoS Pathog 13:e1006379
Rutaganira, Florentine U; Barks, Jennifer; Dhason, Mary Savari et al. (2017) Inhibition of Calcium Dependent Protein Kinase 1 (CDPK1) by Pyrazolopyrimidine Analogs Decreases Establishment and Reoccurrence of Central Nervous System Disease by Toxoplasma gondii. J Med Chem 60:9976-9989
Brown, Kevin M; Long, Shaojun; Sibley, L David (2017) Plasma Membrane Association by N-Acylation Governs PKG Function in Toxoplasma gondii. MBio 8:

Showing the most recent 10 out of 84 publications