Abstract

The current proposal will focus on the mechanisms of hematopoietic reconstitution following drug treatment, and utilize combination anti-retroviral therapy as an adjunct to assess the efficacy of various stem cell genetic therapeutic approaches to combat HIV-induced pathology.
The specific aims i nclude: (1) optimize and further define the loss and subsequent reconstitution of thymopoiesis seen following anti- retroviral treatment of HIV-infected Thy/Liv implants; (2) optimize and further define the mechanisms responsible for loss and subsequent reconstitution of non-lymphoid hematopoietic precursor activity in vivo; and (3) optimize in vivo efficacy of anti-viral gene therapeutic approaches in conjunction with pharmacologic anti-retroviral therapy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI036554-06
Application #
2886944
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Program Officer
Sarver, Nava
Project Start
1994-08-01
Project End
2001-04-30
Budget Start
1999-05-01
Budget End
2000-04-30
Support Year
6
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Gali?, Zoran; Kitchen, Scott G; Subramanian, Aparna et al. (2009) Generation of T lineage cells from human embryonic stem cells in a feeder free system. Stem Cells 27:100-7
Subramanian, Aparna; Guo, Beichu; Marsden, Matthew D et al. (2009) Macrophage differentiation from embryoid bodies derived from human embryonic stem cells. J Stem Cells 4:29-45
Arlen, Philip A; Brooks, David G; Gao, Lian Y et al. (2006) Rapid expression of human immunodeficiency virus following activation of latently infected cells. J Virol 80:1599-603
Galic, Zoran; Kitchen, Scott G; Kacena, Amelia et al. (2006) T lineage differentiation from human embryonic stem cells. Proc Natl Acad Sci U S A 103:11742-7
Chang, Margaret; Brown, Helen J; Collado-Hidalgo, Alicia et al. (2005) beta-Adrenoreceptors reactivate Kaposi's sarcoma-associated herpesvirus lytic replication via PKA-dependent control of viral RTA. J Virol 79:13538-47
Moore, John P; Kitchen, Scott G; Pugach, Pavel et al. (2004) The CCR5 and CXCR4 coreceptors--central to understanding the transmission and pathogenesis of human immunodeficiency virus type 1 infection. AIDS Res Hum Retroviruses 20:111-26
Brooks, David G; Arlen, Philip A; Gao, Lianying et al. (2003) Identification of T cell-signaling pathways that stimulate latent HIV in primary cells. Proc Natl Acad Sci U S A 100:12955-60
Liu, Xuan; Zha, Junli; Nishitani, Junko et al. (2003) HIV-1 infection in peripheral blood lymphocytes (PBLs) exposed to alcohol. Virology 307:37-44
Brooks, David G; Hamer, Dean H; Arlen, Philip A et al. (2003) Molecular characterization, reactivation, and depletion of latent HIV. Immunity 19:413-23
Cole, Steve W; Kemeny, Margaret E; Fahey, John L et al. (2003) Psychological risk factors for HIV pathogenesis: mediation by the autonomic nervous system. Biol Psychiatry 54:1444-56

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