Rapid progress in the areas of molecular virology and molecular epidemiology of human caliciviruses (HuCVs) in recent years highlights the clinical importance and worldwide distribution of HuCVs as a cause of acute gastroenteritis in humans. This renewal builds on the scientific productivity of the past and will initiate new research initiatives based on novel approaches. Our long-term goals are to seak new information about the virology, immunology and pathogenesis of HuCVs and to develop strategies to control and prevent the diseases caused by these viruses. The following four specific aims will be addressed.
Specific aim 1. To define the HuCV antigenic types and develop diagnostic assays for detection and typing using type-common and type-specific antibodies.
Specific aim 2. To characterize the infection status and immunity of children and adults involved in sporadic and outbreak episodes of gastroenteritis using type-common and type-specific assays against HuCVs.
Specific aim 3. To characterize the molecular basis of virus-cell interaction and possible viral receptor(s) using recombinant virus-like particles (VLPs) as a probe.
Specific aim 4. To characterize primate CV (PrCV) replication and attempt to cultivate HuCVs in cell culture using novel approaches learned from animal CVs.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Project (R01)
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Experimental Virology Study Section (EVR)
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Berard, Diana S
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Children's Hospital Med Ctr (Cincinnati)
United States
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Dai, Ying-Chun; Zhang, Xu-Fu; Xia, Ming et al. (2015) Antigenic Relatedness of Norovirus GII.4 Variants Determined by Human Challenge Sera. PLoS One 10:e0124945
Jin, Miao; Tan, Ming; Xia, Ming et al. (2015) Strain-specific interaction of a GII.10 Norovirus with HBGAs. Virology 476:386-94
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Dai, Ying-Chun; Zhang, Xu-Fu; Tan, Ming et al. (2013) A dual chicken IgY against rotavirus and norovirus. Antiviral Res 97:293-300
Fang, Hao; Tan, Ming; Xia, Ming et al. (2013) Norovirus P particle efficiently elicits innate, humoral and cellular immunity. PLoS One 8:e63269
Liu, Yang; Huang, Pengwei; Jiang, Baoming et al. (2013) Poly-LacNAc as an age-specific ligand for rotavirus P[11] in neonates and infants. PLoS One 8:e78113
Yu, Guimei; Zhang, Dongsheng; Guo, Fei et al. (2013) Cryo-EM structure of a novel calicivirus, Tulane virus. PLoS One 8:e59817
Liu, Yang; Huang, Pengwei; Tan, Ming et al. (2012) Rotavirus VP8*: phylogeny, host range, and interaction with histo-blood group antigens. J Virol 86:9899-910

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