Abstract

This project proposes to investigate protein-protein interactions of HIV integrase with host protein. In previous studies, the PI used the yeast two hybrid system to isolate mutants of HIV integrase defective in oligomerization. She has also used the two hybrid system to identify a protein, Ini-1, that binds tightly to HIV-1 integrase. Ini-1 turns out to be similar in sequence to Snf-5, a component of the Swi/Snf complex proposed to be involved in gene activation. The PI plans to investigate heteromeric protein-protein interactions by further experiments using the yeast two hybrid system. Mutants will be generated that influence these interactions, and their phenotypes studied in vitro and in vivo. The influences of Ini-1 on target site selection during retroviral integration will also be investigated, as will the role of Ini-1 in the HIV life cycle. It is hoped that results of these studies will be useful in devising integration inhibitors and in developing new gene therapy methods.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI039951-02
Application #
2429508
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Project Start
1996-06-01
Project End
1999-05-31
Budget Start
1997-06-01
Budget End
1998-05-31
Support Year
2
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Genetics
Type
Schools of Medicine
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461