Abstract

The objective of this proposal is to assess whether the quantity and drug susceptibility of HIV in cervicovaginal secretions correlate with those in plasma among HIV-infected women receiving antiretroviral therapy. The study has important implications for both heterosexual and vertical transmission of HIV. The study will enroll four separate groups of HIV-infected women and follow them prospectively for 36 months. Group A will include 40 women who are antiretroviral naive and who will begin therapy with combination nucleoside reverse transcriptase inhibitor (RTI) therapy. Group B will include 40 women who are already on combination nucleoside RTI, and who will begin a protease inhibitor. Group C will include 20 women who are antiretroviral naive who do not wish to begin therapy. Group D will include 20 recent seroconverters (within one year). HIV load will be measured in plasma and cervicovaginal secretions by NASBA. HIV resistance phenotype will be determined by combined approaches of isolate phenotyping and genotyping of proviral DNA predominately in viral isolates.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
1R01AI040350-01A1
Application #
2330459
Study Section
AIDS and Related Research Study Section 4 (ARRD)
Project Start
1997-04-15
Project End
2002-03-31
Budget Start
1997-04-15
Budget End
1998-03-31
Support Year
1
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Miriam Hospital
Department
Type
DUNS #
039318308
City
Providence
State
RI
Country
United States
Zip Code
02906

  Publications

Venkatesh, Kartik K; DeLong, Allison K; Kantor, Rami et al. (2013) Persistent genital tract HIV-1 RNA shedding after change in treatment regimens in antiretroviral-experienced women with detectable plasma viral load. J Womens Health (Larchmt) 22:330-8
Lahey, Timothy; Ghosh, Mimi; Fahey, John V et al. (2012) Selective impact of HIV disease progression on the innate immune system in the human female reproductive tract. PLoS One 7:e38100
Nijhawan, A E; Delong, A K; Chapman, S et al. (2012) Effect of HSV-2 suppressive therapy on genital tract HIV-1 RNA shedding among women on HAART: a pilot randomized controlled trial. Infect Dis Obstet Gynecol 2012:868526
Mukura, Lucy R; Ghosh, Mimi; Fahey, John V et al. (2012) Genital tract viral load in HIV Type 1-positive women correlates with specific cytokine levels in cervical-vaginal secretions but is not a determinant of infectious virus or anti-HIV activity. AIDS Res Hum Retroviruses 28:1533-9
Ghosh, Mimi; Shen, Zheng; Fahey, John V et al. (2010) Trappin-2/Elafin: a novel innate anti-human immunodeficiency virus-1 molecule of the human female reproductive tract. Immunology 129:207-19
Cu-Uvin, Susan; DeLong, Allison K; Venkatesh, Kartik K et al. (2010) Genital tract HIV-1 RNA shedding among women with below detectable plasma viral load. AIDS 24:2489-97
Ghosh, Mimi; Fahey, John V; Shen, Zheng et al. (2010) Anti-HIV activity in cervical-vaginal secretions from HIV-positive and -negative women correlate with innate antimicrobial levels and IgG antibodies. PLoS One 5:e11366
Mitchell, Caroline; Moreira, Carla; Fredricks, David et al. (2009) Detection of fastidious vaginal bacteria in women with HIV infection and bacterial vaginosis. Infect Dis Obstet Gynecol 2009:236919
Moreira, Carla; Venkatesh, Kartik K; DeLong, Allison et al. (2009) Effect of treatment of asymptomatic bacterial vaginosis on HIV-1 shedding in the genital tract among women on antiretroviral therapy: a pilot study. Clin Infect Dis 49:991-2
Anderson, Brenna L; Wang, Chia-Ching; Delong, Allison K et al. (2008) Genital tract leukocytes and shedding of genital HIV type 1 RNA. Clin Infect Dis 47:1216-21

Showing the most recent 10 out of 18 publications