CD1 molecules are evolutionarily conserved molecules from the immunoglobulin superfamily present in all mammalian species. Distinct from both MHC class I and MHC class II molecules, the CD1 family comprises a third group of MHC-like molecules with diverse functions in both antigen specific and systemic immune responses. CD1 molecules are grouped according to sequence homology into two primary groups: Group one containing human CD1a, b, c and Group two containing human CD1d and mouse Cd1.1 and CD1.2. Unlike MHC class I and II molecules which present peptides to T lymphocytes, CD1 (b and c) has been shown to present lipid glycolipid antigens from Mycobacteria, thus expanding the realm of potential immunogens. CD1 present in mice has been definitively connected to the function of NK1+T cells, which following activation promptly secrete cytokines which can influence the overall dynamics of immune response in both infections and autoimmunity. Additional information is required to understand this complex system which intertwines antigen specific and innate immunity. This proposal addresses three main aspects of the CD1 dependent immune response in mice, with an additional aim designed to expand this information to understanding human Cd1d, of which little is currently known. To understand the role of CD1 in controlling the overall immune system balance and its disruption in autoimmunity, we will utilize CD1 deficient animals and genetic models of diabetes (NOD mice) and lupus (MRL-lpr/lpr mice) in order to analyze the mechanisms of autoimmune suppression. Secondly, we seek to understand the effects of this general immune control on infectious disease, and to determine relative effects of cytokine balance and specialized antigen presentation in mouse models of tuberculosis, leishmaniasis, and helminthic infection.
A third aim i s to reveal the physiological range of antigen specific responses involving CD1, through development of T cells specific for both alloantigens and infectious microbes. Finally, we have generated human CD1d transgenic mice to determine if these molecules can function similarly to human Cd1b and c and/or mouse CD1. Introducing the human CD1d transgene into CD1-deficient mice will allow testing for whether the mouse and human molecules are functionally homologous in the control of NK1+ T cell development and cytokine secretion. The flexibility of analyzing the human molecule in the mouse system will allow dissection of the potential importance of human CD1d in a number of diseases where cytokine balance and specialized antigen presentation affect pathology.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
1R01AI043407-01
Application #
2670858
Study Section
Immunological Sciences Study Section (IMS)
Project Start
1998-08-01
Project End
2002-07-31
Budget Start
1998-08-01
Budget End
1999-07-31
Support Year
1
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Chicago
Department
Pathology
Type
Schools of Medicine
DUNS #
225410919
City
Chicago
State
IL
Country
United States
Zip Code
60637
Bagchi, Sreya; Genardi, Samantha; Wang, Chyung-Ru (2018) Linking CD1-Restricted T Cells With Autoimmunity and Dyslipidemia: Lipid Levels Matter. Front Immunol 9:1616
Weng, Xiufang; He, Ying; Visvabharathy, Lavanya et al. (2017) Crosstalk between type II NKT cells and T cells leads to spontaneous chronic inflammatory liver disease. J Hepatol 67:791-800
Wang, Yajun; Yun, Chawon; Gao, Beixue et al. (2017) The Lysine Acetyltransferase GCN5 Is Required for iNKT Cell Development through EGR2 Acetylation. Cell Rep 20:600-612
O'Hagan, Kyle L; Zhao, Jie; Pryshchep, Olga et al. (2015) Pak2 Controls Acquisition of NKT Cell Fate by Regulating Expression of the Transcription Factors PLZF and Egr2. J Immunol 195:5272-84
Siddiqui, Sarah; Visvabharathy, Lavanya; Wang, Chyung-Ru (2015) Role of Group 1 CD1-Restricted T Cells in Infectious Disease. Front Immunol 6:337
Zhao, Jie; Weng, Xiufang; Bagchi, Sreya et al. (2014) Polyclonal type II natural killer T cells require PLZF and SAP for their development and contribute to CpG-mediated antitumor response. Proc Natl Acad Sci U S A 111:2674-9
Weng, Xiufang; Liao, Chia-Min; Bagchi, Sreya et al. (2014) The adaptor protein SAP regulates type II NKT-cell development, cytokine production, and cytotoxicity against lymphoma. Eur J Immunol 44:3646-57
Zhao, Jie; Bagchi, Sreya; Wang, Chyung-Ru (2014) Type II natural killer T cells foster the antitumor activity of CpG-oligodeoxynucleotides. Oncoimmunology 3:e28977
Liao, Chia-Min; Zimmer, Michael I; Wang, Chyung-Ru (2013) The functions of type I and type II natural killer T cells in inflammatory bowel diseases. Inflamm Bowel Dis 19:1330-8
Liao, Chia-Min; Zimmer, Michael I; Shanmuganad, Sharmila et al. (2012) dysregulation of CD1d-restricted type ii natural killer T cells leads to spontaneous development of colitis in mice. Gastroenterology 142:326-34.e1-2

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