Abstract

The yeast Candida albicans is found as a commensal organism in the digestive tract of mammals. It is also the most common human fungal pathogen, causing both mucosal and systemic'infections, particularlyin immunocompromised people. This proposal covers studies of six different aspects of Candida five of which have been implicated in aspects of pathogenesis. (The sixth has not yet been studied.) These areas are (1) the reversible blastospore to filament morophologica transitions, (2) specialization of hyphal tip cells, (3) white-opaque switching, (4) mating, (5) the identification of 'virulence'genes and, (6) the possible role of regulated RNA splicing in virulence.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI049187-10
Application #
7767722
Study Section
Pathogenic Eukaryotes Study Section (PTHE)
Program Officer
Duncan, Rory A
Project Start
2001-03-01
Project End
2011-11-30
Budget Start
2010-03-01
Budget End
2011-11-30
Support Year
10
Fiscal Year
2010
Total Cost
$618,582
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Dalal, Chiraj K; Johnson, Alexander D (2017) How transcription circuits explore alternative architectures while maintaining overall circuit output. Genes Dev 31:1397-1405
Craik, Veronica B; Johnson, Alexander D; Lohse, Matthew B (2017) Sensitivity of White and Opaque Candida albicans Cells to Antifungal Drugs. Antimicrob Agents Chemother 61:
Lohse, Matthew B; Ene, Iuliana V; Craik, Veronica B et al. (2016) Systematic Genetic Screen for Transcriptional Regulators of the Candida albicans White-Opaque Switch. Genetics 203:1679-92
Lohse, Matthew B; Johnson, Alexander D (2016) Identification and Characterization of Wor4, a New Transcriptional Regulator of White-Opaque Switching. G3 (Bethesda) 6:721-9
Dalal, Chiraj K; Zuleta, Ignacio A; Mitchell, Kaitlin F et al. (2016) Transcriptional rewiring over evolutionary timescales changes quantitative and qualitative properties of gene expression. Elife 5:
Hernday, Aaron D; Lohse, Matthew B; Nobile, Clarissa J et al. (2016) Ssn6 Defines a New Level of Regulation of White-Opaque Switching in Candida albicans and Is Required For the Stochasticity of the Switch. MBio 7:e01565-15
Lohse, Matthew B; Kongsomboonvech, Pisiwat; Madrigal, Maria et al. (2016) Genome-Wide Chromatin Immunoprecipitation in Candida albicans and Other Yeasts. Methods Mol Biol 1361:161-84
Ene, Iuliana V; Lohse, Matthew B; Vladu, Adrian V et al. (2016) Phenotypic Profiling Reveals that Candida albicans Opaque Cells Represent a Metabolically Specialized Cell State Compared to Default White Cells. MBio 7:
Du, Han; Guan, Guobo; Li, Xiaoling et al. (2015) N-Acetylglucosamine-Induced Cell Death in Candida albicans and Its Implications for Adaptive Mechanisms of Nutrient Sensing in Yeasts. MBio 6:e01376-15
Mancera, Eugenio; Porman, Allison M; Cuomo, Christina A et al. (2015) Finding a Missing Gene: EFG1 Regulates Morphogenesis in Candida tropicalis. G3 (Bethesda) 5:849-56

Showing the most recent 10 out of 46 publications