: Plasmodium sporozoites migrate through and invade different cell types in the mosquito vector and vertebrate host. At the end of their journey they transform into exo-erythrocytic forms within a hepatocyte. Irradiated sporozoites induce protective immunity against malaria and therefore it is of great importance to identify sporozoite and EEF proteins entering the host hepatocyte. However, little is known about secreted and surface proteins of Plasmodium sporozoites and their involvement in sporozoite-host cell interactions. No information is available about proteins involved in the cross talk of the intracellular EEF with the host hepatocyte. The overall objective of this proposal is the characterization of newly identified sporozoite-expressed and EEF-expressed proteins and their role in host-parasite interactions and the identification of additional molecules that could be important for these interactions. This will lead to a more complete understanding of the parasites complex biology but might also identify new potential candidates for inclusion into a pre-erythrocytic subunit vaccine and targets for transmission blocking strategies.
| Arredondo, Silvia A; Kappe, Stefan H I (2017) The s48/45 six-cysteine proteins: mediators of interaction throughout the Plasmodium life cycle. Int J Parasitol 47:409-423 |
| Groat-Carmona, Anna M; Kain, Heather; Brownell, Jessica et al. (2015) A Plasmodium ?/?-hydrolase modulates the development of invasive stages. Cell Microbiol 17:1848-67 |
| Lindner, Scott E; Swearingen, Kristian E; Harupa, Anke et al. (2013) Total and putative surface proteomics of malaria parasite salivary gland sporozoites. Mol Cell Proteomics 12:1127-43 |
| Pei, Ying; Miller, Jessica L; Lindner, Scott E et al. (2013) Plasmodium yoelii inhibitor of cysteine proteases is exported to exomembrane structures and interacts with yoelipain-2 during asexual blood-stage development. Cell Microbiol 15:1508-1526 |
| Vaughan, Ashley M; Kappe, Stefan H I; Ploss, Alexander et al. (2012) Development of humanized mouse models to study human malaria parasite infection. Future Microbiol 7:657-65 |
| Lindner, Scott E; Miller, Jessica L; Kappe, Stefan H I (2012) Malaria parasite pre-erythrocytic infection: preparation meets opportunity. Cell Microbiol 14:316-24 |
| MacKellar, Drew C; Vaughan, Ashley M; Aly, Ahmed S I et al. (2011) A systematic analysis of the early transcribed membrane protein family throughout the life cycle of Plasmodium yoelii. Cell Microbiol 13:1755-67 |
| Jacobs-Lorena, Vanessa Y; Mikolajczak, Sebastian A; Labaied, Mehdi et al. (2010) A dispensable Plasmodium locus for stable transgene expression. Mol Biochem Parasitol 171:40-4 |
| Kappe, Stefan H I; Vaughan, Ashley M; Boddey, Justin A et al. (2010) That was then but this is now: malaria research in the time of an eradication agenda. Science 328:862-6 |
| Vaughan, Ashley M; Wang, Ruobing; Kappe, Stefan H I (2010) Genetically engineered, attenuated whole-cell vaccine approaches for malaria. Hum Vaccin 6:107-13 |
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