Abstract

Having determined the X-ray crystal structures of Glycera and lamprey hemoglobins at 2.5 and 2.0 Angstrom units respectively, it is proposed to refine these structures both with and without ligands at 1.5 Angstrom units resolution.
The aim i s to define as objectively and precisely as possible the structural changes that accompany the binding of ligands. Thus, details will be obtained about the stereochemical events that occur in these heme pockets and additional data will become available to complement that already known from Chironomus hemoglobin, sperm whale myoglobin and human hemoglobin. It is proposed to determine the crystal structure of polymeric lamprey hemoglobin, complete the low resolution structures of a subunit of Limulus hemocyanin and liganded Trout hemoglobins I and IV.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM002528-25
Application #
3150691
Study Section
Biophysics and Biophysical Chemistry B Study Section (BBCB)
Project Start
1977-09-01
Project End
1986-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
25
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
Schools of Arts and Sciences
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Royer Jr, W E; Hendrickson, W A; Love, W E (1987) Crystals of Lumbricus erythrocruorin. J Mol Biol 197:149-53
Honzatko, R B; Hendrickson, W A (1986) Molecular models for the putative dimer of sea lamprey hemoglobin. Proc Natl Acad Sci U S A 83:8487-91
Royer Jr, W E; Love, W E; Fenderson, F F (1985) Cooperative dimeric and tetrameric clam haemoglobins are novel assemblages of myoglobin folds. Nature 316:277-80
Honzatko, R B; Hendrickson, W A; Love, W E (1985) Refinement of a molecular model for lamprey hemoglobin from Petromyzon marinus. J Mol Biol 184:147-64