We propose to develop membrane-cell fusion methods to test for thyroid stimulating immunoglobulins (TSI) and for cell-mediated cytotoxicity: 1) Using TSH-receptors from fat or thyroid cell membranes fused into cultured mouse tumor cells, develop sensitive TSH and TSI assay, measuring cAMP response. 2) Characterize biochemically the thyroglobulin-binding protein(s) in human eye muscle; transfer this material to lipid vesicles, and fuse the latter to 111In-cultured tumor cells. Test cytotoxicity of recipient cells exposed to human lymphocytes of Graves' disease patients. We will conduct a 12 year follow-up review of the results of orbital radiotherapy in 200 patients with Graves' ophthalmopathy.
| Tao, T W; Cheng, P J; Pham, H et al. (1986) Monoclonal antithyroglobulin antibodies derived from immunizations of mice with human eye muscle and thyroid membranes. J Clin Endocrinol Metab 63:577-82 |
| Tao, T W; Gatenby, P A; Leu, S L et al. (1985) Helper and suppressor activities of lymphocyte subsets on antithyroglobulin production in vitro. J Clin Endocrinol Metab 61:520-4 |
| Tao, T W; Leu, S L; Kriss, J P (1985) Peripheral blood lymphocytes from normal individuals can be induced to secrete immunoglobulin G antibodies against self-antigen thyroglobulin in vitro. J Clin Endocrinol Metab 60:279-82 |
| Austin-Seymour, M M; Donaldson, S S; Egbert, P R et al. (1985) Radiotherapy of lymphoid diseases of the orbit. Int J Radiat Oncol Biol Phys 11:371-9 |
