Abstract

The overall objective of this research grant is to study the participation of sterol carrier proteins in cholesterol biosynthesis, utilization and intracellular transfer.
Specific aims related to the structure of sterol carrier proteins include the purification of sterol carrier protein2 (SCP2) from rat, pig and human tissues, the preparation on anti-SCP2 IgG, peptide mapping of SCP2, determination of the amino acid sequence of SCP2, and a detailed structural comparison of SCP2 to several other lipid transfer protein preparations.
Specific aims related to the function of sterol carrier proteins include studies on the participation of SCP1 and SCP2 in cholesterol biosynthesis, the participation of SCP2 in intracellular cholesterol transport in rat liver, the participation of SCP(2) in the enzyme cholesterol 7Lambda-hydroxylase, the participation of SCP(2) in adrenal cholesterol transport and steroid hormone biosynthesis, functional comparisons of SCP(2) to various lipid transfer proteins, and regulatory interactions of cholesterol SCP(2) with the enzyme system which phosphorylates HMG-CoA reductase, the major rate limiting enzyme in cholesterol biosynthesis. A better understanding of the participation of sterol carrier proteins in cholesterol biosynthesis, utilization and intracellular transfer should lead to a better understanding of cholesterol metabolism in health and disease.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM010628-20
Application #
3150784
Study Section
(SSS)
Project Start
1978-05-01
Project End
1988-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
20
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of New Mexico
Department
Type
Schools of Medicine
DUNS #
829868723
City
Albuquerque
State
NM
Country
United States
Zip Code
87131

  Publications

Knight, G D; Laubscher, K H; Fore, M L et al. (1994) Vitalethine modulates erythropoiesis and neoplasia. Cancer Res 54:5623-35
Knight, G D; Mann, P L; Laubscher, K H et al. (1994) Seemingly diverse activities of beta-alethine. Cancer Res 54:5636-42
Schroeder, F; Butko, P; Nemecz, G et al. (1990) Interaction of fluorescent delta 5,7,9(11),22-ergostatetraen-3 beta-ol with sterol carrier protein-2. J Biol Chem 265:151-7
Schroeder, F; Butko, P; Hapala, I et al. (1990) Intermembrane cholesterol transfer: role of sterol carrier proteins and phosphatidylserine. Lipids 25:669-74
Trzeciak, W H; Simpson, E R; Scallen, T J et al. (1987) Studies on the synthesis of sterol carrier protein-2 in rat adrenocortical cells in monolayer culture. Regulation by ACTH and dibutyryl cyclic 3',5'-AMP. J Biol Chem 262:3713-7
Pastuszyn, A; Noland, B J; Bazan, J F et al. (1987) Primary sequence and structural analysis of sterol carrier protein 2 from rat liver: homology with immunoglobulins. J Biol Chem 262:13219-27
Vahouny, G V; Chanderbhan, R; Kharroubi, A et al. (1987) Sterol carrier and lipid transfer proteins. Adv Lipid Res 22:83-113
Chanderbhan, R F; Kharroubi, A T; Noland, B J et al. (1986) Sterol carrier protein2: further evidence for its role in adrenal steroidogenesis. Endocr Res 12:351-70
Scallen, T J; Pastuszyn, A; Noland, B J et al. (1985) Sterol carrier and lipid transfer proteins. Chem Phys Lipids 38:239-61
Seltman, H; Diven, W; Rizk, M et al. (1985) Regulation of bile-acid synthesis. Role of sterol carrier protein 2 in the biosynthesis of 7 alpha-hydroxycholesterol. Biochem J 230:19-24

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