Abstract

The proposed study is designed to investigate the chemical nature and biosynthesis of epidermal cell components (i.e., keratohyalin granules, nucleus) observed morphologically under normal, experimental and pathological conditions, in order to better understand biochemical changes involved in each stage of epidermal cell differentiation (the """"""""keratinization"""""""" process). Factors influencing the process will be investigated and elucidation of control mechanisms is sought. Cytochemical, autoradiographical, chemical and immunological techniques will be used. These results will clarify pathomechanisms of skin diseases associated with abnormal keratinization and contribute to the control of these diseases.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM012433-18
Application #
3150827
Study Section
General Medicine A Subcommittee 2 (GMA)
Project Start
1976-04-15
Project End
1986-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
18
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Harvima, R J; Yabe, K; Fraki, J E et al. (1988) Hydrolysis of histones by proteinases. Biochem J 250:859-64
Harvima, R J; Yabe, K; Fraki, J E et al. (1987) Separation and identification of cathepsins in newborn rat epidermis. J Invest Dermatol 88:393-7
Toku, S; Fukuyama, K; Epstein, W L (1987) Purification and some properties of bound cysteine proteinase inhibitor from cornified cells of newborn rat epidermis. Biochim Biophys Acta 925:48-56
Nakano, S; Fukuyama, K; Epstein, W L (1987) The highly modified membrane of cornified cells in stratified squamous epithelia: a comparison of heterogeneous deposits in keratinized and nonkeratinized epithelia. Cell Tissue Res 249:331-6
Hara, A; Kato, T; Sawada, H et al. (1985) Characterization of Fe2+-activated acid phosphatase in rat epidermis. Comp Biochem Physiol B 82:269-74
Fukuyama, K; Ito, Y; Yabe, K et al. (1985) Immunological detection of a cysteine protease in the skin and other tissues. Cell Tissue Res 240:417-23
Asahi, M; Lindquist, R; Fukuyama, K et al. (1985) Purification and characterization of major extracellular proteinases from Trichophyton rubrum. Biochem J 232:139-44
Schneider, S A; Fukuyama, K; Maceira, J et al. (1985) Effect of ultraviolet B radiation on S-100 protein antigen in epidermal Langerhans cells. J Invest Dermatol 84:146-8