Abstract

It is proposed to study: (1) properties of adrenodoxin, adrenodoxin reductase, and their complex, (2) purification and properties of cytochrome P450scc and P45011 Beta from bovine adrenocortical mitochondria, (3) substrate availability for cholesterol side chain cleavage reaction of adrenocortical mitochondria, (4) Incorporation of P450scc into artificial phospholipid vesicles. The method of procedure used in this proposal are extensive purification and characterization of enzymes and proteins. By means of reconstitution of these components into a system close to that in the native system of adrenal cortex, we hope to be able to understand the relationship between the steroidogenesis and the action of ACTH.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM012713-17
Application #
3150836
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1976-05-01
Project End
1987-08-31
Budget Start
1985-09-01
Budget End
1987-08-31
Support Year
17
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Wayne State University
Department
Type
Schools of Arts and Sciences
DUNS #
City
Detroit
State
MI
Country
United States
Zip Code
48202

  Publications

Hara, T; Kimura, T (1989) Purification and catalytic properties of a cross-linked complex between adrenodoxin reductase and adrenodoxin. J Biochem 105:594-600
Hara, T; Kimura, T (1989) Active complex between adrenodoxin reductase and adrenodoxin in the cytochrome P-450scc reduction reaction. J Biochem 105:601-5
Hayashi, Y; Kimura, T (1986) Vanadium inhibits ACTH-mediated but not cyclic AMP-dependent adrenal steroidogenesis. Mol Cell Endocrinol 44:17-24
Huang, Y Y; Hara, T; Sligar, S et al. (1986) Thermodynamic properties of oxidation-reduction reactions of bacterial, microsomal, and mitochondrial cytochromes P-450: an entropy-enthalpy compensation effect. Biochemistry 25:1390-4
Kobayashi, S; Nakano, M; Goto, T et al. (1986) An evidence of the peroxidase-dependent oxygen transfer from hydrogen peroxide to sulfides. Biochem Biophys Res Commun 135:166-71
Hayashi, Y; Kimura, T (1986) The effects of vanadium compounds on the activation of adenylate cyclase from rat adrenal membrane. Biochim Biophys Acta 869:29-36
Igarashi, Y; Kimura, T (1986) Importance of the unsaturated fatty acyl group of phospholipids in their stimulatory role on rat adrenal mitochondrial steroidogenesis. Biochemistry 25:6461-6
Igarashi, Y; Kimura, T (1986) Adrenic acid content in rat adrenal mitochondrial phosphatidylethanolamine and its relation to ACTH-mediated stimulation of cholesterol side chain cleavage reaction. J Biol Chem 261:14118-24
Kimura, T (1986) Transduction of ACTH signal from plasma membrane to mitochondria in adrenocortical steroidogenesis. Effects of peptide, phospholipid, and calcium. J Steroid Biochem 25:711-6
Watabe, S; Kimura, T (1985) Adrenal cortex mitochondrial enzyme with ATP-dependent protease and protein-dependent ATPase activities. Purification and properties. J Biol Chem 260:14498-504

Showing the most recent 10 out of 14 publications