Abstract

We are studying the mechanisms by which experimental diabetes alters the function of the intestinal brush border membrane. We have previously shown that the stimulation of intestinal sucrase activity occurs because of impairment of enzyme protein degradation. During the next year we will study the lipid composition of the brush border membrane in an effort to explain the altered degradation of enzyme protein.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM013927-16
Application #
3150894
Study Section
(GCN)
Project Start
1976-06-15
Project End
1987-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
16
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
Schools of Medicine
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Lloyd, M; Mevissen, G; Fischer, M et al. (1992) Regulation of intestinal lactase in adult hypolactasia. J Clin Invest 89:524-9
Li, B; Lloyd, M L; Gudjonsson, H et al. (1992) The effect of enteral carnitine administration in humans. Am J Clin Nutr 55:838-45
Li, B U; Bummer, P M; Hamilton, J W et al. (1990) Uptake of L-carnitine by rat jejunal brush border microvillous membrane vesicles. Evidence of passive diffusion. Dig Dis Sci 35:333-9
Witte, J; Lloyd, M; Lorenzsonn, V et al. (1990) The biosynthetic basis of adult lactase deficiency. J Clin Invest 86:1338-42
Burke, T; Lloyd, M; Lorenzsonn, V et al. (1988) Synthesis and intracellular processing of aminooligopeptidase by human intestine. Gastroenterology 94:1426-31
Lloyd, M L; Olsen, W A (1987) A study of the molecular pathology of sucrase-isomaltase deficiency. A defect in the intracellular processing of the enzyme. N Engl J Med 316:438-42
Lorenzsonn, V; Korsmo, H; Olsen, W A (1987) Localization of sucrase-isomaltase in the rat enterocyte. Gastroenterology 92:98-105
Olsen, W A; Perchellet, E; Malinowski, R L (1986) Intestinal mucosa in diabetes: synthesis of total proteins and sucrase-isomaltase. Am J Physiol 250:G788-93
Hamilton, J W; Li, B U; Shug, A L et al. (1986) Carnitine transport in human intestinal biopsy specimens. Demonstration of an active transport system. Gastroenterology 91:10-6
Gudjonsson, H; Li, B U; Shug, A L et al. (1985) In vivo studies of intestinal carnitine absorption in rats. Gastroenterology 88:1880-7

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