Abstract

The purpose of the studies of red cell and granulocyte membranes, outlined in this proposal, is to define changes in cell membrane protein and lipid metabolism which may influence cell flexibility and permeability. The flexibility and permeability of erythrocytes is crucial to cell survival in vivo and is also particularly relevant to the problem of prolonged erythrocyte storage. Particular attention is focused upon the role of the Red cell membrane protein Spectrin since defects in Spectrin appear to be a common denominator in several clinical Hemolytic disorders.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM016095-14
Application #
3150990
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1977-08-01
Project End
1987-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
14
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Low, P S; Willardson, B M; Mohandas, N et al. (1991) Contribution of the band 3-ankyrin interaction to erythrocyte membrane mechanical stability. Blood 77:1581-6
Chasis, J A; Schrier, S L (1989) Membrane deformability and the capacity for shape change in the erythrocyte. Blood 74:2562-8
Galili, U; Mandrell, R E; Hamadeh, R M et al. (1988) Interaction between human natural anti-alpha-galactosyl immunoglobulin G and bacteria of the human flora. Infect Immun 56:1730-7
Shiffer, K A; Goerke, J; Duzgunes, N et al. (1988) Interaction of erythrocyte protein 4.1 with phospholipids. A monolayer and liposome study. Biochim Biophys Acta 937:269-80
Galili, U; Basbaum, C B; Shohet, S B et al. (1987) Identification of erythrocyte Gal alpha 1-3Gal glycosphingolipids with a mouse monoclonal antibody, Gal-13. J Biol Chem 262:4683-8
Chasis, J A; Shohet, S B (1987) Red cell biochemical anatomy and membrane properties. Annu Rev Physiol 49:237-48
Lorand, L; Michalska, M; Murthy, S N et al. (1987) Cross-linked polymers in the red cell membranes of a patient with Hb-Koln disease. Biochem Biophys Res Commun 147:602-7
Galili, U; Buehler, J; Shohet, S B et al. (1987) The human natural anti-Gal IgG. III. The subtlety of immune tolerance in man as demonstrated by crossreactivity between natural anti-Gal and anti-B antibodies. J Exp Med 165:693-704
Chasis, J A; Mohandas, N (1986) Erythrocyte membrane deformability and stability: two distinct membrane properties that are independently regulated by skeletal protein associations. J Cell Biol 103:343-50
Galili, U; Clark, M R; Shohet, S B (1986) Excessive binding of natural anti-alpha-galactosyl immunoglobin G to sickle erythrocytes may contribute to extravascular cell destruction. J Clin Invest 77:27-33

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