Abstract

The purpose of this research proposal is to continue the study of normal and pathological articular and epiphyseal cartilage with special attention to the metabolims of the tissue in health and a variety of disease states, especially human osteoarthritis. The studies to be performed include a continued study of DNA replicative activity in normal and pathological cartilages with special emphasis on the use of flow cytometry; a continued definition of the products and rates of synthesis of normal and pathologic cartilages utilizing a variety of biochemical and metabolic techniques including the cell-free systems; a continued study of a variety of normal and pathological control mechanisms of the metabolic (both anabolic and catabolic) activities of normal and abnormal cartilages; a search for intracellular and cell membrane markers of disease states in normal and pathologic cartilages, chiefly by use of the flow cytometer; and continued attempts at isolation, identification, purification and determination of optimal conditions, substrate, cofactors and inhibitors for degradative enzymes in normal and diseased articular and epiphyseal cartilages.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM016265-14
Application #
3151002
Study Section
General Medicine A Subcommittee 2 (GMA)
Project Start
1977-04-01
Project End
1988-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
14
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code

  Publications

Lewis, V O; Gehrmann, M; Weissbach, L et al. (1999) Homologous plasminogen N-terminal and plasminogen-related gene A and B peptides. Characterization of cDNAs and recombinant fusion proteins. Eur J Biochem 259:618-25
Weissbach, L; Treadwell, B V (1992) A plasminogen-related gene is expressed in cancer cells. Biochem Biophys Res Commun 186:1108-14
Lalanandham, T; Ehrlich, M G; Zaleske, D J et al. (1990) Viability and metabolism of cartilage transplanted to physeal regions. J Pediatr Orthop 10:450-8
Diao, E; Zaleske, D J; Avella, D et al. (1989) Kinetic and biochemical heterogeneity in vertebrate chondroepiphyseal regions during development. J Orthop Res 7:502-10
Towle, C A; Trice, M E; Ollivierre, F et al. (1987) Regulation of cartilage remodeling by IL-1: evidence for autocrine synthesis of IL-1 by chondrocytes. J Rheumatol 14 Spec No:11-3
Ollivierre, F; Gubler, U; Towle, C A et al. (1986) Expression of IL-1 genes in human and bovine chondrocytes: a mechanism for autocrine control of cartilage matrix degradation. Biochem Biophys Res Commun 141:904-11
Nojima, T; Towle, C A; Mankin, H J et al. (1986) Secretion of higher levels of active proteoglycanases from human osteoarthritic chondrocytes. Arthritis Rheum 29:292-5
Treadwell, B V; Mankin, H J (1986) The synthetic processes of articular cartilage. Clin Orthop Relat Res :50-61
Treadwell, B V; Towle, C A; Ishizue, K et al. (1986) Stimulation of the synthesis of collagenase activator protein in cartilage by a factor present in synovial-conditioned medium. Arch Biochem Biophys 251:724-31
Madreperla, S A; Louwerenburg, B; Mann, R W et al. (1985) Induction of heat-shock protein synthesis in chondrocytes at physiological temperatures. J Orthop Res 3:30-5

Showing the most recent 10 out of 12 publications