Abstract

The proposed research is aimed at elucidation of relationships which may exist between variations in plasma membrane calcium transport and the expression of certain human diseases. The red blood cell will be used. Major emphasis will be on clarification of the regulation of calcium transport in normal human cells and possible variation(s) sickle cell anemia, cystic fibrosis, and muscular dystrophy. The calcium pump is based on a membrane-bound (Ca2 ion plus Mg2 ion)-ATPase. Calmodulin, which is a protein present in red blood cells, increases the activity of the calcium pump ATPase. The hypothesis to be tested is that the calmodulin acts as a switch which turns on the plasma membrane calcium pump. It is anticipated that results of the proposed research will provide further information on the plasma membrane calcium transport system in normal cells and variations which exist in certain diseases.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM016436-10
Application #
3151008
Study Section
(EH)
Project Start
1978-09-30
Project End
1987-06-30
Budget Start
1985-06-01
Budget End
1987-06-30
Support Year
10
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Hinds, T R; Hammond, W P; Maggio-Price, L et al. (1989) The activity of the red blood cell Ca pump is decreased in hemolytic anemia of the beagle dog. Blood Cells 15:407-20;discussion 421-6
Maggio-Price, L; Emerson, C L; Hinds, T R et al. (1988) Hereditary nonspherocytic hemolytic anemia in beagles. Am J Vet Res 49:1020-5
Hinds, T R; DiJulio, D; Vincenzi, F F (1987) Red blood cell Ca2+ pump ATPase: inhibition by compound 48/80. Proc West Pharmacol Soc 30:93-5
Dodson, R A; Vincenzi, F F (1987) Preliminary evidence for decreased red blood cell deformability during blood storage. Proc West Pharmacol Soc 30:117-9
Dodson, R A; Hinds, T R; Vincenzi, F F (1987) Effects of calcium and A23187 on deformability and volume of human red blood cells. Blood Cells 12:555-64
Vincenzi, F F; Morris, C D; Kinsel, L B et al. (1986) Decreased calcium pump adenosine triphosphatase in red blood cells of hypertensive subjects. Hypertension 8:1058-66
Hinds, T R; Vincenzi, F F (1986) Evidence for a calmodulin-activated Ca2+ pump ATPase in dog erythrocytes. Proc Soc Exp Biol Med 181:542-9
Hinds, T R; Vincenzi, F F (1985) The effect of ETH 1001 on ion fluxes across red blood cell membranes. Cell Calcium 6:265-79
Schmidt, J W; Hinds, T R; Vincenzi, F F (1985) On the failure of calmodulin to activate Ca2+ pump ATPase of dog red blood cells. Comp Biochem Physiol A Comp Physiol 82:601-7