Abstract

The proposed work is in four parts: a) the preparation and examination by electron microscopy of transporting epithelia, b) the use of computer-assisted morphometric procedures to measure cell dimensions and subsequently to construct quantitative three-dimensional models of cell shape, c) the phenomenological description of relationships between epithelial transport function and the shape of epithelial cells, and d) physiological experimentation on isolated epithelia to measure directly functional parameters predicted by the structure-function correlation studies. To date, morphologic studies on five different segments of the adult rabbit nephron have been completed and structure-function correlations have been established for three of those segments. Studies are nearly complete on the other two adult nephron segments and on the proximal nephron of newborn rabbit, adult nephron segments under varying experimental conditions, rabbit gallbladder, and frogs nephron. A computer model of cell membrane porosity under steady-state and transient flow conditions also has been completed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM016968-13
Application #
3151040
Study Section
General Medicine B Study Section (GMB)
Project Start
1977-08-01
Project End
1986-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
13
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Kansas
Department
Type
Schools of Medicine
DUNS #
016060860
City
Kansas City
State
KS
Country
United States
Zip Code
66160

  Publications

Linshaw, M A; Macalister, T J; Welling, L W et al. (1991) Role of cytoskeleton in isotonic cell volume control of rabbit proximal tubules. Am J Physiol 261:F60-9
Welling, L W; Evan, A P; Gattone 2nd, V H et al. (1989) Correlation of structure and function in developing proximal tubule of guinea pig. Am J Physiol 256:F13-7
Furuse, A; Bernstein, J; Welling, L W et al. (1989) Renal tubular differentiation in mouse and mouse metanephric culture. I. Ultrastructural studies. Pediatr Nephrol 3:265-72
Carey, H V; Cooke, H J; Gerthoffer, W T et al. (1989) Intestinal transport in megacolonic mice. Alterations in sugar absorption. Dig Dis Sci 34:185-92
Furuse, A; Bernstein, J; Welling, L W et al. (1989) Renal tubular differentiation in mouse and mouse metanephric culture. II. Na-K-ATPase activity. Pediatr Nephrol 3:273-9
Welling, L W; Linshaw, M A (1988) Structural and functional development of outer versus inner cortical proximal tubules. Pediatr Nephrol 2:108-14
Welling, L W; Welling, D J (1988) Relationship between structure and function in renal proximal tubule. J Electron Microsc Tech 9:171-85
Welling, D J; Welling, L W (1988) Model of renal cell volume regulation without active transport: role of a heteroporous membrane. Am J Physiol 255:F529-38
Welling, L W; Welling, D J; Ochs, T J (1987) Video measurement of basolateral NaCl reflection coefficient in proximal tubule. Am J Physiol 253:F290-8
Welling, L W; Welling, D J; Holsapple, J W et al. (1987) Morphometric analysis of distinct microanatomy near the base of proximal tubule cells. Am J Physiol 253:F126-40

Showing the most recent 10 out of 13 publications