Abstract

The long term goals of this study on basement membrane structure are the determination of the chemical and physical properties of polypeptide components and the elucidation of their organization and mode of interaction. The basement membranes to be studied are the glomerular basement membrane from bovine kidneys, lens capsule and Descemet's basement membrane from bovine eyes, and intestinal basement membrane from Ascaris suum. The methods to be used include gel filtration, ion-exchange, gas-liquid, and high pressure liquid chromatography, analytical ultracentrifugation, electrophoresis, circular dichroism spectroscopy, and chemical analyses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM018381-12
Application #
3151137
Study Section
Pathobiochemistry Study Section (PBC)
Project Start
1978-02-01
Project End
1986-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
12
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Kansas
Department
Type
Schools of Medicine
DUNS #
016060860
City
Kansas City
State
KS
Country
United States
Zip Code
66160

  Publications

Zhang, X; Hudson, B G; Sarras Jr, M P (1994) Hydra cell aggregate development is blocked by selective fragments of fibronectin and type IV collagen. Dev Biol 164:10-23
Langeveld, J P; Noelken, M E; Hard, K et al. (1991) Bovine glomerular basement membrane. Location and structure of the asparagine-linked oligosaccharide units and their potential role in the assembly of the 7 S collagen IV tetramer. J Biol Chem 266:2622-31
Langeveld, J P; Wieslander, J; Timoneda, J et al. (1988) Structural heterogeneity of the noncollagenous domain of basement membrane collagen. J Biol Chem 263:10481-8
Butkowski, R J; Langeveld, J P; Wieslander, J et al. (1987) Localization of the Goodpasture epitope to a novel chain of basement membrane collagen. J Biol Chem 262:7874-7
Wieslander, J; Kataja, M; Hudson, B G (1987) Characterization of the human Goodpasture antigen. Clin Exp Immunol 69:332-40
Noelken, M E; Wisdom Jr, B J; Dean, D C et al. (1986) Intestinal basement membrane of Ascaris suum. Molecular organization and properties of the collagen molecules. J Biol Chem 261:4706-14
Butkowski, R J; Wieslander, J; Wisdom, B J et al. (1985) Properties of the globular domain of type IV collagen and its relationship to the Goodpasture antigen. J Biol Chem 260:3739-47
Wieslander, J; Langeveld, J; Butkowski, R et al. (1985) Physical and immunochemical studies of the globular domain of type IV collagen. Cryptic properties of the Goodpasture antigen. J Biol Chem 260:8564-70