In our studies to date we have (1) identified receptors for lactogen, insulin, gorwth hormone, and somatomedins (IGFs) in Golgi elements from rodent liver; and shown that intracellular and cell surface receptors are regulated differently. (2) Identified a unique intracellular vesicle, other than classical Golgi, enriched in hormone receptors. (3) Studied internalization into pancreatic acinar cells wherein we have shown intracellular radiolabeled insulin associated with secretory (anabolic) elements of the vacuolar system. (4) Initiated studies on the mechanism of chloroquine action. (5) Obtained evidence for the intracellular translocation (processing) of hormone. In the coming year we plan to: (1) Isolate and further characterize the unique intracellular vesicle. (2) Study factors regulating intracellular processing of hormone. (3) Determine the precise site of chloroquine action. (4) Study the internalization of glucagon and parathyroid hormone. (5) Start studies on the sequence of metabolism of internalized insulin and on the possible biological actions of internalized hormone.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM019573-08
Application #
3151230
Study Section
Metabolism Study Section (MET)
Project Start
1977-05-01
Project End
1987-01-31
Budget Start
1985-02-01
Budget End
1986-01-31
Support Year
8
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Mcgill University
Department
Type
DUNS #
City
Montreal
State
PQ
Country
Canada
Zip Code
H3 2T5
Dorato, A; Raguet, S; Okamura, H et al. (1992) Characterization of the structure and glycosylation properties of intracellular and cell surface rat hepatic prolactin receptors. Endocrinology 131:1734-42
Walsh, R J; Slaby, F J; Posner, B I (1987) A receptor-mediated mechanism for the transport of prolactin from blood to cerebrospinal fluid. Endocrinology 120:1846-50
Silverman, W F; Walsh, R J; Posner, B I (1986) The ontogeny of specific prolactin binding sites in the rat choroid plexus. Brain Res 389:11-9