Abstract

The nuclear matrix of the mammalian nucleus has been isolated and characterized. This cellular structural component appears to be involved in the nuclear functions such as DNA synthesis. The nuclear matrix of the immature rat uterus has high affinity, saturable estrogen binding sites which appears to account for a considerable portion of the salt resistant nuclear estrogen receptors reported by others. The nuclear matrix of the uterus and prostate will be characterized and studied for their binding properties with sex steroid and antisteroid compounds. The nuclear matrix may be associated with a residual structural component extending throughout the cell and is termed the cell matrix. Steroid binding to these components will also be examined.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM022000-14
Application #
3151427
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1978-09-01
Project End
1988-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
14
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Pienta, K J; Partin, A W; Coffey, D S (1989) Cancer as a disease of DNA organization and dynamic cell structure. Cancer Res 49:2525-32
Mohler, J L; Partin, A W; Lohr, W D et al. (1988) Nuclear roundness factor measurement for assessment of prognosis of patients with prostatic carcinoma. I. Testing of a digitization system. J Urol 139:1080-4
Mohler, J L; Partin, A W; Epstein, J I et al. (1988) Nuclear roundness factor measurement for assessment of prognosis of patients with prostatic carcinoma. II. Standardization of methodology for histologic sections. J Urol 139:1085-90
Alexander, R B; Nelson, W G; Coffey, D S (1987) Synergistic enhancement by tumor necrosis factor of in vitro cytotoxicity from chemotherapeutic drugs targeted at DNA topoisomerase II. Cancer Res 47:2403-6
Nelson, W G; Coffey, D S (1987) Structural aspects of mammalian DNA replication: topoisomerase II. NCI Monogr :23-9
Nelson, W G; Cho, K R; Hsiang, Y H et al. (1987) Growth-related elevations of DNA topoisomerase II levels found in Dunning R3327 rat prostatic adenocarcinomas. Cancer Res 47:3246-50
Alexander, R B; Greene, G L; Barrack, E R (1987) Estrogen receptors in the nuclear matrix: direct demonstration using monoclonal antireceptor antibody. Endocrinology 120:1851-7
Nelson, W G; Pienta, K J; Barrack, E R et al. (1986) The role of the nuclear matrix in the organization and function of DNA. Annu Rev Biophys Biophys Chem 15:457-75
Nelson, W G; Liu, L F; Coffey, D S (1986) Newly replicated DNA is associated with DNA topoisomerase II in cultured rat prostatic adenocarcinoma cells. Nature 322:187-9
Alexander, R B; Isaacs, W B; Barrack, E R (1985) Immunogold probes for electron microscopy: evaluation of staining by fluorescence microscopy. J Histochem Cytochem 33:995-1000