Abstract

The specific aims of this proposal are as follows: 1) To construct a cDNA library from the mRNAs of a number of homozygous lymphoblastoid cell lines and clone full-length cDNA copies in E. coli, to sequence the cDNA inserts and from them deduce the amino acid sequences of a number of HLA-A, -B, and -C antigen alleles; 2) To isolate genomic clones from human genomic libraries established from homozygous lymphoblastoid cell lines and to analyze these genomic clones and their flanking regions, to study expression of the genomic clones in several systems with a view to using this technique in identification of the clones; 3) To attempt to obtain cDNA clones corresponding to the heavy and light chains of HLA-DR antigens and of the human equivalent of the murine TL and Qa antigens; 4) To study at the genetic level the relationship between a number of human diseases and particular antigens in the HLA region, particularly ankylosing spondylitis (HLA-B27, hereditary hemochromatosis (HLA-A3), multiple sclerosis (HLA-DR), juvenile onset diabetes (HLA-DR3 and 4) and rheumatoid arthritis (HLA-DR4).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM030241-04
Application #
3152033
Study Section
Mammalian Genetics Study Section (MGN)
Project Start
1982-01-01
Project End
1986-12-31
Budget Start
1985-01-01
Budget End
1985-12-31
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Harvard University
Department
Type
Schools of Arts and Sciences
DUNS #
071723621
City
Cambridge
State
MA
Country
United States
Zip Code

  Publications

Hata, S; Satyanarayana, K; Devlin, P et al. (1988) Extensive junctional diversity of rearranged human T cell receptor delta genes. Science 240:1541-4
Haas, D A; Boss, J M; Strominger, J L et al. (1987) A highly diverged beta 1 exon in the DR region of the human MHC: sequence and evolutionary implications. Immunogenetics 25:15-20
Miwa, K; Doyle, C; Strominger, J L (1987) Sequence-specific interactions of nuclear factors with conserved sequences of human class II major histocompatibility complex genes. Proc Natl Acad Sci U S A 84:4939-43
Boss, J M; Strominger, J L (1986) Regulation of a transfected human class II major histocompatibility complex gene in human fibroblasts. Proc Natl Acad Sci U S A 83:9139-43
Prentice, H L; Schneider, P M; Strominger, J L (1986) C4B gene polymorphism detected in a human cosmid clone. Immunogenetics 23:274-6
Sorrentino, R; Auffray, C; Boss, J et al. (1986) Major histocompatibility complex class II antigens: genes and proteins. Mt Sinai J Med 53:202-9
Barbosa, J A; Mentzer, S J; Kamarck, M E et al. (1986) Gene mapping and somatic cell hybrid analysis of the role of human lymphocyte function-associated antigen-3 (LFA-3) in CTL-target cell interactions. J Immunol 136:3085-91
Quertermous, T; Murre, C; Dialynas, D et al. (1986) Human T-cell gamma chain genes: organization, diversity, and rearrangement. Science 231:252-5
Spies, T; Morton, C C; Nedospasov, S A et al. (1986) Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex. Proc Natl Acad Sci U S A 83:8699-702
Strominger, J L (1986) Human major histocompatibility complex genes: class I antigens and tumor necrosis factors. Cold Spring Harb Symp Quant Biol 51 Pt 1:63-6

Showing the most recent 10 out of 19 publications