Abstract

The proposed research is aimed at bridging the gap of knowledge between the biochemistry, ultrastructure and cell physiology of cell structure and motility especially in amoeboid cells. The investigations center around functional cell extracts from amoeboid cells which can be regulated to exhibit gelation-solation and contraction. Specific proteins will be isolated from the extracts and the interaction of these proteins with actin and myosin will be investigated by standard solution biochemical techniques and fluorescence spectroscopy. Finally, the distribution and interaction of these fluorescently labeled cytoskeletal and contractile proteins will be studied in living cells using our technique of Molecular Cytochemistry. Quantitative spectroscopic techniques will also be tested on living cells using a microspectrofluorometer.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM032461-04
Application #
3152533
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1982-07-01
Project End
1986-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Carnegie-Mellon University
Department
Type
Schools of Arts and Sciences
DUNS #
052184116
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Hahn, K M; Waggoner, A S; Taylor, D L (1990) A calcium-sensitive fluorescent analog of calmodulin based on a novel calmodulin-binding fluorophore. J Biol Chem 265:20335-45
Galbraith, W; Ryan, K W; Gliksman, N et al. (1989) Multiple spectral parameter imaging in quantitative fluorescence microscopy. I: Quantitation of bead standards. Comput Med Imaging Graph 13:47-60
Bright, G R; Whitaker, J E; Haugland, R P et al. (1989) Heterogeneity of the changes in cytoplasmic pH upon serum stimulation of quiescent fibroblasts. J Cell Physiol 141:410-9
Bright, G R; Fisher, G W; Rogowska, J et al. (1989) Fluorescence ratio imaging microscopy. Methods Cell Biol 30:157-92
Luby-Phelps, K; Lanni, F; Taylor, D L (1988) The submicroscopic properties of cytoplasm as a determinant of cellular function. Annu Rev Biophys Biophys Chem 17:369-96
DeBiasio, R L; Wang, L L; Fisher, G W et al. (1988) The dynamic distribution of fluorescent analogues of actin and myosin in protrusions at the leading edge of migrating Swiss 3T3 fibroblasts. J Cell Biol 107:2631-45
Simon, J R; Furukawa, R H; Ware, B R et al. (1988) The molecular mobility of alpha-actinin and actin in a reconstituted model of gelation. Cell Motil Cytoskeleton 11:64-82
Luby-Phelps, K; Taylor, D L (1988) Subcellular compartmentalization by local differentiation of cytoplasmic structure. Cell Motil Cytoskeleton 10:28-37
Simon, J R; Gough, A; Urbanik, E et al. (1988) Analysis of rhodamine and fluorescein-labeled F-actin diffusion in vitro by fluorescence photobleaching recovery. Biophys J 54:801-15
Bright, G R; Fisher, G W; Rogowska, J et al. (1987) Fluorescence ratio imaging microscopy: temporal and spatial measurements of cytoplasmic pH. J Cell Biol 104:1019-33

Showing the most recent 10 out of 16 publications