Abstract

This proposal is aimed at studying the long-term culture of human and rat functional pituitary tumor cells and somatic cell hybrids secreting growth hormone or prolactin. Hormone modulation by thyrotropin-releasing hormone (TRH), growth hormone releasing factor (GRF), thyroid hormones and other neurotransmitters will be studied. The human pituitary cell cultures will provide an in vitro model for studying mechanisms of hormone synthesis and release and provide further insights into the regulation of human prolactin and growth hormone secretion, the pathophysiology of pituitary tumors and female and male reproductive control and infertility.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM033802-02
Application #
3152956
Study Section
Endocrinology Study Section (END)
Project Start
1984-09-30
Project End
1987-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Cedars-Sinai Medical Center
Department
Type
DUNS #
075307785
City
Los Angeles
State
CA
Country
United States
Zip Code
90048

  Publications

Siegel, R J; Fishbein, M C; Said, J W et al. (1989) Identification of growth hormone at the myocardial cell surface. Am J Cardiovasc Pathol 2:345-50
Fagin, J A; Melmed, S (1987) Relative increase in insulin-like growth factor I messenger ribonucleic acid levels in compensatory renal hypertrophy. Endocrinology 120:718-24
Pixley, S; Weiss, M; Melmed, S (1987) Identification of human growth hormone messenger ribonucleic acid in pituitary adenoma cells by in situ hybridization. J Clin Endocrinol Metab 65:575-80
Fagin, J A; Pixley, S; Slanina, S et al. (1987) Insulin-like growth factor I gene expression in GH3 rat pituitary cells: messenger ribonucleic acid content, immunocytochemistry, and secretion. Endocrinology 120:2037-43
Melmed, S; Yamashita, S; Kovacs, K et al. (1987) Cushing's syndrome due to ectopic proopiomelanocortin gene expression by islet cell carcinoma of the pancreas. Cancer 59:772-8
Yamashita, S; Melmed, S (1987) Insulinlike growth factor I regulation of growth hormone gene transcription in primary rat pituitary cells. J Clin Invest 79:449-52
Melmed, S; Fagin, J A (1987) Acromegaly update--etiology, diagnosis and management. West J Med 146:328-36
Melmed, S; Fagin, J A (1986) Isolation and characterization of rat-mouse somatic cell hybrids secreting growth hormone and prolactin. Exp Cell Res 162:475-85
Melmed, S; Braunstein, G D; Chang, R J et al. (1986) Pituitary tumors secreting growth hormone and prolactin. Ann Intern Med 105:238-53
Karpf, D B; Yamashita, S; Melmed, S et al. (1986) In vitro secretion of deoxycorticosterone by a benign Leydig cell tumor of the testis. J Urol 136:114-6

Showing the most recent 10 out of 22 publications