1. To use rat monoclonal antibodies to the rat """"""""I-A(u)"""""""" and """"""""I-E(u)"""""""" class II major histocompatibility complex antigens to prevent insulin dependent diabetes mellitus (IDDM) in the BB/Worcester rat. 2. To produce rat monoclonal antibodies to the rat islet cell surface antigen(s) (ICSA) expressed on insulin producing Beta cells. 3. To use monoclonal Alpha-ICSA antibodies to isolate and characterize rat ICSA. 4. To use affinity purified ICSA to prevent IDDM in the BB/Worcester rat. 5. To use purified ICSA to develop a rapid assay for Alpha-ICSA antibody. 6. To clone and sequence the cDNA sequences encoding the variant sub-types of HLA-DR4Beta, HLA-DR3Beta, HLA""""""""DC4""""""""Beta, and HLA-""""""""DC3""""""""Beta.
| Acha-Orbea, H; McDevitt, H O (1987) The first external domain of the nonobese diabetic mouse class II I-A beta chain is unique. Proc Natl Acad Sci U S A 84:2435-9 |
