Calcium movements during E-C coupling in giant muscle fibers from the barnacle will be followed using the aequorin technique. Calcium transients will be quantified by the use of calcium sensitive electrodes. The characteristic relationship between voltage clamped membrane potential and the release of calcium from the sarcoplasmic reticulum will be determined. The relationship between calcium level and force will be found using a calcium clamp to control the level of calcium activation. Small length changes will be used to delineate the characteristics of the extra calcium seen during crossbridge cycling. The aequorin technique will be used to investigate the mechanism of sperm activation, and the role of calcium transients during cell division in Medaka eggs.
| Gordon, A M; Ridgway, E B (1993) Cross-bridges affect both TnC structure and calcium affinity in muscle fibers. Adv Exp Med Biol 332:183-92;discussion 192-4 |
| Gordon, A M; Ridgway, E B (1990) Stretch of active muscle during the declining phase of the calcium transient produces biphasic changes in calcium binding to the activating sites. J Gen Physiol 96:1013-35 |
| Gordon, A M; Ridgway, E B; Yates, L D et al. (1988) Muscle cross-bridge attachment: effects on calcium binding and calcium activation. Adv Exp Med Biol 226:89-99 |
| Gordon, A M; Ridgway, E B (1987) Extra calcium on shortening in barnacle muscle. Is the decrease in calcium binding related to decreased cross-bridge attachment, force, or length? J Gen Physiol 90:321-40 |
| Levine, M A; Morrow, P P; Kronenberg, H M et al. (1986) Tissue and gene specific hypomethylation of the human parathyroid hormone gene: association with parathyroid hormone gene expression in parathyroid glands. Endocrinology 119:1618-24 |
