Abstract

Collagen is the major structural protein of the body and recent studies have emphasized its role in other biological processes including differentiation and cell adhesion. Connective tissue abnormalities which may be related to biological properties of collagen occur throughout medicine. These include fibrotic conditions, osteoporosis, wound healing, developmental defects, and aging. Human skin fibroblasts in culture devote a considerable portion of their biosynthetic capacities to collagen production and provide a convenient means for studying the regulation of its synthesis. Collagen synthesis including type-specific synthesis and levels of modifying enzymes (lysyl hydroxylase and prolyl hydroxylase) will be studied. An attempt will be made to understand the role and level of regulatory influences including hormonal and nutritional on collagen production in these cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR028304-06
Application #
3155632
Study Section
General Medicine A Subcommittee 2 (GMA)
Project Start
1981-04-01
Project End
1988-06-30
Budget Start
1987-07-01
Budget End
1988-06-30
Support Year
6
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Duke University
Department
Type
Schools of Medicine
DUNS #
071723621
City
Durham
State
NC
Country
United States
Zip Code
27705

  Publications

Yeowell, H N; Marshall, M K; Walker, L C et al. (1994) Regulation of lysyl oxidase mRNA in dermal fibroblasts from normal donors and patients with inherited connective tissue disorders. Arch Biochem Biophys 308:299-305
Murad, S; Walker, L C; Tajima, S et al. (1994) Minimum structural requirements for minoxidil inhibition of lysyl hydroxylase in cultured fibroblasts. Arch Biochem Biophys 308:42-7
Phillips, C L; Combs, S B; Pinnell, S R (1994) Effects of ascorbic acid on proliferation and collagen synthesis in relation to the donor age of human dermal fibroblasts. J Invest Dermatol 103:228-32
Handa, J T; Murad, S; Jaffe, G J (1993) Minoxidil inhibits ocular cell proliferation and lysyl hydroxylase activity. Invest Ophthalmol Vis Sci 34:567-75
Halperin, E C; Gaspar, L; George, S et al. (1993) A double-blind, randomized, prospective trial to evaluate topical vitamin C solution for the prevention of radiation dermatitis. CNS Cancer Consortium. Int J Radiat Oncol Biol Phys 26:413-6
Yeowell, H N; Ha, V; Walker, L C et al. (1992) Characterization of a partial cDNA for lysyl hydroxylase from human skin fibroblasts;lysyl hydroxylase mRNAs are regulated differently by minoxidil derivatives and hydralazine. J Invest Dermatol 99:864-9
Phillips, C L; Tajima, S; Pinnell, S R (1992) Ascorbic acid and transforming growth factor-beta 1 increase collagen biosynthesis via different mechanisms: coordinate regulation of pro alpha 1(I) and Pro alpha 1(III) collagens. Arch Biochem Biophys 295:397-403
Murad, S; Tennant, M C; Pinnell, S R (1992) Structure-activity relationship of minoxidil analogs as inhibitors of lysyl hydroxylase in cultured fibroblasts. Arch Biochem Biophys 292:234-8
Darr, D; Combs, S; Dunston, S et al. (1992) Topical vitamin C protects porcine skin from ultraviolet radiation-induced damage. Br J Dermatol 127:247-53
Phillips, C L; Lever, L W; Pinnell, S R et al. (1991) Construction of a full-length murine pro alpha 2(I) collagen cDNA by the polymerase chain reaction. J Invest Dermatol 97:980-4

Showing the most recent 10 out of 12 publications