The objective of this proposal is to characterize the biologic attributes of the dendritic Thy-1 antigen-bearing cells which populate mouse epidermis (EC (Thy-1+)). Focusing first on their cellular attributes we plan to: 1a) refine methods for obtaining cell populations highly enriched for and highly depleted of them, 1b) attempt to propagate them in tissue culture with specialized growth media, 1c) obtain stable cell lines through cell fusion and viral transformation, 2) complete the phenotypic characterization of EC(Thy-1+) in adult epidermis and to compare these attributes with those of other related epidermal cells, 3) assess the ontogeny and migratory patterns of EC(Thy-1+) during the perinatal period after skin grafting, and under the influence of specific drugs (a phorbol ester and corticosteroids), and physical maneuvers (stratum corneum removal and UVB radiation), and 4) test a series of hypotheses concerning functional attributes of EC(Thy-1+), including a. They are T lymphocytes, b. They are non-T lymphocytes, c. They are antigen presenting cells. Functional properties will be tested in vitro and with in vivo studies in which EC(Thy-1+) are purified, treated, and reintroduced by various routes into naive recipients.
| Matsushima, Hironori; Ogawa, Yasushi; Miyazaki, Toru et al. (2010) Intravital imaging of IL-1beta production in skin. J Invest Dermatol 130:1571-80 |
| Matsushima, Hironori; Takashima, Akira (2010) Bidirectional homing of Tregs between the skin and lymph nodes. J Clin Invest 120:653-6 |
| Mayuzumi, Nobuyasu; Matsushima, Hironori; Takashima, Akira (2009) IL-33 promotes DC development in BM culture by triggering GM-CSF production. Eur J Immunol 39:3331-42 |
| Matsushima, Hironori; Tanaka, Hiroaki; Mizumoto, Norikatsu et al. (2009) Identification of crassin acetate as a new immunosuppressant triggering heme oxygenase-1 expression in dendritic cells. Blood 114:64-73 |
| Mizumoto, Norikatsu; Tanaka, Hiroaki; Matsushima, Hironori et al. (2007) Colchicine promotes antigen cross-presentation by murine dendritic cells. J Invest Dermatol 127:1543-6 |
| Ward, Brant R; Jester, James V; Nishibu, Akiko et al. (2007) Local thermal injury elicits immediate dynamic behavioural responses by corneal Langerhans cells. Immunology 120:556-72 |
| Ogawa, Yasushi; Mizumoto, Norikatsu; Tanaka, Hiroaki et al. (2006) Identification of novel pharmacological activities of an antifungal agent, nystatin, to promote dendritic cell maturation. J Invest Dermatol 126:349-53 |
| Mizumoto, Norikatsu; Hui, Francis; Edelbaum, Dale et al. (2005) Differential activation profiles of multiple transcription factors during dendritic cell maturation. J Invest Dermatol 124:718-24 |
| Mizumoto, Norikatsu; Gao, Jimin; Matsushima, Hironori et al. (2005) Discovery of novel immunostimulants by dendritic-cell-based functional screening. Blood 106:3082-9 |
| Matsue, Hiroyuki; Matsue, Keiko; Edelbaum, Dale et al. (2004) New strategy for efficient selection of dendritic cell-tumor hybrids and clonal heterogeneity of resulting hybrids. Cancer Biol Ther 3:1145-51 |
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