Sequence Studies on Calcium Binding Proteins

Collins, John H.

Abstract

The amino acid sequences of the following proteins will be determined: the catalytic subunit and proteolipid component of lamb kidney Na, K-ATPase, and a scallop muscle Ca-binding protein. The techniques used and results obtained in the sequence studies will be applied to structure-function studies on membrane and contractile proteins.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
• Type: Research Project (R01)
• Project #: 3R01AR035120-02S1
• Application #: 3157039
• Study Section: Pathobiochemistry Study Section (PBC)
• Project Start: 1984-07-01
• Project End: 1987-06-30
• Budget Start: 1986-07-01
• Budget End: 1987-06-30
• Support Year: 2
• Fiscal Year: 1986

Institution

• Name: Clarkson University
• Type: Schools of Arts and Sciences
• DUNS #: 041590993
• City: Potsdam
• State: NY
• Country: United States
• Zip Code: 13699

Publications

Barouch, W W; Breese, K E; Davidoff, S A et al. (1991) Amino acid sequences of myosin essential and regulatory light chains from two clam species: comparison with other molluscan myosin light chains. J Muscle Res Cell Motil 12:321-32

Leszyk, J; Collins, J H; Leavis, P C et al. (1988) Cross-linking of rabbit skeletal muscle troponin subunits: labeling of cysteine-98 of troponin C with 4-maleimidobenzophenone and analysis of products formed in the binary complex with troponin T and the ternary complex with troponins I and T. Biochemistry 27:6983-7

Collins, J H; Cox, J A; Theibert, J L (1988) Amino acid sequence of a sarcoplasmic calcium-binding protein from the sandworm Nereis diversicolor. J Biol Chem 263:15378-85

Leszyk, J; Dumaswala, R; Potter, J D et al. (1988) Amino acid sequence of bovine cardiac troponin I. Biochemistry 27:2821-7

Leszyk, J; Dumaswala, R; Potter, J D et al. (1987) Bovine cardiac troponin T: amino acid sequences of the two isoforms. Biochemistry 26:7035-42

Leszyk, J; Collins, J H; Leavis, P C et al. (1987) Cross-linking of rabbit skeletal muscle troponin with the photoactive reagent 4-maleimidobenzophenone: identification of residues in troponin I that are close to cysteine-98 of troponin C. Biochemistry 26:7042-7

Collins, J H; Leszyk, J (1987) The ""gamma subunit"" of Na,K-ATPase: a small, amphiphilic protein with a unique amino acid sequence. Biochemistry 26:8665-8