Lyme disease is caused by the tick borne spirochete Borrelia burgdorferi and there are estimated to be 300,000 cases/year in the US. It is associated with a spectrum of clinical symptoms of varying severities, ranging from localized skin lesion to disseminated infection including secondary skin lesions, arthritis, neuropathies, and carditis. It is apparent that genetically regulated responses of the host contribute to the severity of symptoms. We have taken a forward genetics approach to identify allelic genes that determine the difference in Lyme arthritis between the severely arthritic C3H mouse and the mildly arthritic C57BL/6 (B6) mouse. This analysis has identified Quantitative Trait Loci on several chromosomes, including the Bbaa1 locus on Chr 4, which encompasses the Type I IFN gene cluster. Congenic mouse lines in which the C3H Bbaa1 allele was introgressed onto the B6 background (B6.C3H-Bbaa1) confirmed the presence of a genetic element in this locus that regulates arthritis through expression of IFN?, a Type I IFN. As the C3H and B6 IFN? genes lack polymorphisms, any expression differences must be regulated by a linked genetic element. Advanced, interval specific recombinant congenic lines (ISRCL) have narrowed the physical region associated with Bbaa1 to 2.2 Mbp and identified the Cyclin Dependent Kinase Inhibitory gene, Cdkn2a, as a candidate regulator of IFN? expression and determinant of the severity of Lyme arthritis. A variety of experiments demonstrated that the Bbaa1 impact on IFN? expression was manifest through B. burgdorferi stimulation of myeloid cells, but that an additional, downstream mediator of arthritis required distinct activation of resident cells of the joint tissues. Independent studies characterized the arthritis-inducing activity of IFN? as myostatin (MSTN). MSTN is well characterize as modulating skeletal muscle development, but has also been implicated in chronic disease and inflammation. We will utilize the B6.C3-Bbaa1 ISRCLs to characterize the mechanism by which products of the Cdkn2a locus regulate IFN? production in myeloid cells, and, in turn, the mechanism by which the downstream effector MSTN modulates Lyme arthritis development. We propose a combination of lineage-specific expression analysis, silencing of gene expression, development of targeted KO mice, and the use of small molecule inhibitors in order to define the complex interactions involved in Lyme arthritis development. Our findings may provide insight into other pathologies driven by Type I IFN, which could also implicate unexpected pathways involving Cdkn2a and MTSN.
Lyme disease, caused by the spirochete Borrelia burgdorferi, is associated with a spectrum of clinical symptoms and a dramatic range in severity among infected individuals. A forward genetics approach identified a locus on Chr 4 of the mouse that regulates Lyme arthritis by controlling the expression of Type I IFN, and downstream induction of MSTN as an effector of arthritis development. The mechanism by which Type I IFN and MSTN contribute to Lyme arthritis development will be addressed, with the goal of identifying novel therapeutic targets for patients with severe disease.
Showing the most recent 10 out of 50 publications