Abstract

This is a renewal of a long-standing study of growth regulation in estrogen target cells. The grant would extend ongoing studies to determine how serum proteins inhibit growth of mammary cells in culture.
Three specific aims are presented which would identify the specific regions of serum albumin which block growth of estrogen sensitive cells, characterize the active fraction of serum from albumin negative patients, and finally, assess the role of a serum albumin receptor in the mammary cells which might explain the effects of the growth repressor in serum.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA013410-25
Application #
6375563
Study Section
Special Emphasis Panel (ZRG2-MEP (04))
Program Officer
Sathyamoorthy, Neeraja
Project Start
1995-09-30
Project End
2003-04-30
Budget Start
2001-05-01
Budget End
2002-04-30
Support Year
25
Fiscal Year
2001
Total Cost
$293,196
Indirect Cost

  Institution

Name
Tufts University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02111

  Publications

Soto, Ana M; Maffini, Maricel V; Schaeberle, Cheryl M et al. (2006) Strengths and weaknesses of in vitro assays for estrogenic and androgenic activity. Best Pract Res Clin Endocrinol Metab 20:15-33
Soto, Ana M; Sonnenschein, Carlos (2004) The somatic mutation theory of cancer: growing problems with the paradigm? Bioessays 26:1097-107
Powell, Charles E; Soto, Ana M; Michaelson, Cheryl L et al. (2003) Characterization of a plasma membrane-resident albumin-binding protein associated with the proliferation of estrogen-target, serum-sensitive cells. Steroids 68:487-96
Maffini, Maricel V; Geck, Peter; Powell, Charles E et al. (2002) Mechanism of androgen action on cell proliferation: AS3 protein as a mediator of proliferative arrest in the rat prostate. Endocrinology 143:2708-14
Powell, C E; Soto, A M; Sonnenschein, C (2001) Identification and characterization of membrane estrogen receptor from MCF7 estrogen-target cells. J Steroid Biochem Mol Biol 77:97-108
Sonnenschein, C; Soto, A M (2000) Somatic mutation theory of carcinogenesis: why it should be dropped and replaced. Mol Carcinog 29:205-11
Geck, P; Maffini, M V; Szelei, J et al. (2000) Androgen-induced proliferative quiescence in prostate cancer cells: the role of AS3 as its mediator. Proc Natl Acad Sci U S A 97:10185-90
Szelei, J; Soto, A M; Geck, P et al. (2000) Identification of human estrogen-inducible transcripts that potentially mediate the apoptotic response in breast cancer. J Steroid Biochem Mol Biol 72:89-102
Geck, P; Szelei, J; Jimenez, J et al. (1999) Early gene expression during androgen-induced inhibition of proliferation of prostate cancer cells: a new suppressor candidate on chromosome 13, in the BRCA2-Rb1 locus. J Steroid Biochem Mol Biol 68:41-50
Soto, A M; Michaelson, C L; Prechtl, N V et al. (1998) Assays to measure estrogen and androgen agonists and antagonists. Adv Exp Med Biol 444:9-23;discussion 23-8

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