Abstract

The objective of this work is to identify the gene or genes responsible for the target cell specificity and transforming potential of hematopoietic cells by murine leukemia viruses (MuLV's). We will concentrate on the study of mink cell focus-inducing viruses (MCF's) and the defective spleen focus-forming viruses (SFFV's). We will study the target cell specificity for transformation by using: 1) pseudotypes containing the gemone of a virus with one target cell specificity and the coat from a virus with another target cell specificity; 2) recombinant viruses formed in tissue culture between a virus of one tropism and a virus of another propism; 3) recombinant viruses obtained by ligation of portions of cloned viral DNA's derived from viruses of different target cell specificity; 4) recombinant viruses obtained by marker rescue. We will study the transforming ability and the target cell specificity of the four different components of an in vivo passaged Rauscher-MuLV complex by in vivo and in vitro assay.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA013608-13
Application #
3163811
Study Section
Virology Study Section (VR)
Project Start
1978-05-01
Project End
1986-04-30
Budget Start
1985-05-01
Budget End
1986-04-30
Support Year
13
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Salk Institute for Biological Studies
Department
Type
DUNS #
005436803
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Tran, Tram Anh; Kinch, Lisa; Peña-Llopis, Samuel et al. (2013) Platelet-derived growth factor/vascular endothelial growth factor receptor inactivation by sunitinib results in Tsc1/Tsc2-dependent inhibition of TORC1. Mol Cell Biol 33:3762-79
Kefas, Benjamin; Comeau, Laurey; Floyd, Desiree H et al. (2009) The neuronal microRNA miR-326 acts in a feedback loop with notch and has therapeutic potential against brain tumors. J Neurosci 29:15161-8
Vogt, M; Haggblom, C; Yeargin, J et al. (1998) Independent induction of senescence by p16INK4a and p21CIP1 in spontaneously immortalized human fibroblasts. Cell Growth Differ 9:139-46
Vogt, M; Haggblom, C; Swift, S (1989) Growth factor independence and indefinite growth (""immortalization"") appear simultaneously after crisis in murine myelocytes expressing v-myc. Oncogene Res 4:19-28
Bogenberger, J; Haas, M (1988) cDNA clones from autocrine thymic lymphoma cells encode two mitogenic proteins, a serine protease and a truncated T-cell receptor beta-chain. Oncogene Res 3:301-12
Vogt, M; Lesley, J; Bogenberger, J M et al. (1987) The induction of growth factor-independence in murine myelocytes by oncogenes results in monoclonal cell lines and is correlated with cell crisis and karyotypic instability. Oncogene Res 2:49-63
Vogt, M; Lesley, J; Bogenberger, J et al. (1986) Coinfection with viruses carrying the v-Ha-ras and v-myc oncogenes leads to growth factor independence by an indirect mechanism. Mol Cell Biol 6:3545-9
Vogt, M; Haggblom, C; Swift, S et al. (1986) Specific sequences of the env gene determine the host range of two XC-negative viruses of the Rauscher virus complex. Virology 154:420-4
Vogt, M; Haggblom, C; Swift, S et al. (1985) Envelope gene and long terminal repeat determine the different biological properties of Rauscher, Friend, and Moloney mink cell focus-inducing viruses. J Virol 55:184-92
Mally, M I; Vogt, M; Swift, S E et al. (1985) Oncogene expression in murine splenic T cells and in murine T-cell neoplasms. Virology 144:115-26