Abstract

The aims of this proposal are to complete a description of transcription unit design in eukaryotic cells by determining how and where RNA polymerase II (pol II) transcription ceases. The laboratory is in the process of shifting mainly to transcriptional control studies of pol II transcription units during differentiation. The material to be used is chiefly differentiating erythroblastic (erythroleukemia) cells in culture and normal fetal and adult liver cells. A program is described for the isolation and characterization of genes that are expressed primarily or exclusively in the liver followed by testing which of the sequences confer liver specificity when these genes are reintroduced into animals. Finally, various techniques for following liver specific characteristics will be applied to developing mice to track at a molecular level the earliest detectable markers for liver differentiation. The health relevance of basic studies of this type lies in the confidence that many diseases result from defects in gene regulation both in adult and developing tissues. (G)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA016006-15
Application #
3164327
Study Section
Molecular Biology Study Section (MBY)
Project Start
1979-04-01
Project End
1989-06-30
Budget Start
1988-02-01
Budget End
1989-06-30
Support Year
15
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Rockefeller University
Department
Type
Graduate Schools
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065

  Publications

Song, Jing; Du, Zhanwen; Ravasz, Mate et al. (2015) A Protein Interaction between ?-Catenin and Dnmt1 Regulates Wnt Signaling and DNA Methylation in Colorectal Cancer Cells. Mol Cancer Res 13:969-81
Song, J; Wang, Z; Ewing, R M (2014) Integrated analysis of the Wnt responsive proteome in human cells reveals diverse and cell-type specific networks. Mol Biosyst 10:45-53
Mellacheruvu, Dattatreya; Wright, Zachary; Couzens, Amber L et al. (2013) The CRAPome: a contaminant repository for affinity purification-mass spectrometry data. Nat Methods 10:730-6
Duncan, S A; Zhong, Z; Wen, Z et al. (1997) STAT signaling is active during early mammalian development. Dev Dyn 208:190-8
Weinstein, D C; Ruiz i Altaba, A; Chen, W S et al. (1994) The winged-helix transcription factor HNF-3 beta is required for notochord development in the mouse embryo. Cell 78:575-88
Duncan, S A; Manova, K; Chen, W S et al. (1994) Expression of transcription factor HNF-4 in the extraembryonic endoderm, gut, and nephrogenic tissue of the developing mouse embryo: HNF-4 is a marker for primary endoderm in the implanting blastocyst. Proc Natl Acad Sci U S A 91:7598-602
Chen, W S; Manova, K; Weinstein, D C et al. (1994) Disruption of the HNF-4 gene, expressed in visceral endoderm, leads to cell death in embryonic ectoderm and impaired gastrulation of mouse embryos. Genes Dev 8:2466-77
Zhong, W; Mirkovitch, J; Darnell Jr, J E (1994) Tissue-specific regulation of mouse hepatocyte nuclear factor 4 expression. Mol Cell Biol 14:7276-84
Zhong, W; Sladek, F M; Darnell Jr, J E (1993) The expression pattern of a Drosophila homolog to the mouse transcription factor HNF-4 suggests a determinative role in gut formation. EMBO J 12:537-44
Mirkovitch, J; Darnell Jr, J E (1992) Mapping of RNA polymerase on mammalian genes in cells and nuclei. Mol Biol Cell 3:1085-94

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