Abstract

This project deals with molecular mechanisms underlying Avian Leukosis Virus (ALV) induced neoplasms including bursal lymphomas in chickens. The integrated exogenous provirus of ALV and adjacent host sequences in clonal neoplasms will be cloned in prokaryotic vectors using recombinant DNA technology and detailed studies of the structure and function of these sequences will be carried out. Evidence of recombination with host cell sequences will be sought and integration site of ALV provirus in different normal and neoplastic tissues will be compared. The sequences in cellular DNA preparation from ALV induced neoplasms which transform NIH/3T3 mouse cells in transfection experiments will be characterized with respect to known putative transforming genes in chicken cells and an attempt made to isolate these sequences by molecular cloning techniques. The genetic elements of ALV responsible for its oncongenic properties will be identified by biochemical substitution of allelic segments of a non-oncogenic endogenous avian retrovirus.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA020068-10
Application #
3165229
Study Section
Virology Study Section (VR)
Project Start
1976-03-01
Project End
1986-02-28
Budget Start
1985-03-01
Budget End
1986-02-28
Support Year
10
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
075524595
City
Seattle
State
WA
Country
United States
Zip Code
98109

  Publications

Neiman, Paul E; Elsaesser, Katrina; Loring, Gilbert et al. (2008) Myc oncogene-induced genomic instability: DNA palindromes in bursal lymphomagenesis. PLoS Genet 4:e1000132
Kimmel, Robert R; Agnani, Shivali; Yang, Yin et al. (2008) DNA copy-number instability in low-dose gamma-irradiated TK6 lymphoblastoid clones. Radiat Res 169:259-69
Kimmel, Robert R; Zhao, Lue Ping; Nguyen, Doan et al. (2006) Microarray comparative genomic hybridization reveals genome-wide patterns of DNA gains and losses in post-Chernobyl thyroid cancer. Radiat Res 166:519-31
Neiman, P E; Kimmel, R; Icreverzi, A et al. (2006) Genomic instability during Myc-induced lymphomagenesis in the bursa of Fabricius. Oncogene 25:6325-35
Neiman, Paul E; Burnside, Joan; Elsaesser, Katrina et al. (2006) Analysis of gene expression, copy number and palindrome formation with a Dt40 enriched cDNA microarray. Subcell Biochem 40:245-56
Burnside, Joan; Neiman, Paul; Tang, Jianshan et al. (2005) Development of a cDNA array for chicken gene expression analysis. BMC Genomics 6:13
Brown, Cheryl Y; Bowers, Sandra J; Loring, Gibert et al. (2004) Role of Mtd/Bok in normal and neoplastic B-cell development in the bursa of Fabricius. Dev Comp Immunol 28:619-34
Parghi, Sean S; Brandvold, Kimberly A; Bowers, Sandra J et al. (2004) Reduced Myc overexpression and normal B-cell differentiation mediate resistance to avian leukosis virus lymphomagenesis. Oncogene 23:4413-21
Black, Elizabeth J; Clair, Timothy; Delrow, Jeffrey et al. (2004) Microarray analysis identifies Autotaxin, a tumour cell motility and angiogenic factor with lysophospholipase D activity, as a specific target of cell transformation by v-Jun. Oncogene 23:2357-66
Cheng, Chun; Kimmel, Robert; Neiman, Paul et al. (2003) Array rank order regression analysis for the detection of gene copy-number changes in human cancer. Genomics 82:122-9

Showing the most recent 10 out of 42 publications