The objective of this research is the investigation of the role of DNA repair in radiation-induced mutagenesis and transformation in a mammalian cell line. The effects of stationary holding recovery on the frequencies of these processes is currently under investigation. The characteristics of DNA repair in the cells will be studied by measuring error-free and error prone reactivation of irradiated virus by the host cells under various conditions. We also hope to isolate repair-defective strains following mutagenesis of the cells with ethylmethane sulfonate (EMS) and enrichment using a host-cell viral reactivation suicide technique. Changes in the frequencies of carcinogenesis and mutagenesis in the wild-type cells are compared to the repair-deficient strains will then be correlated to deficiencies in specific repair processes.
Evans, H H (1994) Failla Memorial Lecture. The prevalence of multilocus lesions in radiation-induced mutants. Radiat Res 137:131-44 |
Evans, H H; Horng, M F (1988) The repair of potentially lethal damage and sublethal damage in strains of mouse L5178Y lymphoma cells differing in radiation sensitivity. Radiat Res 113:183-90 |
Evans, H H; Ricanati, M; Horng, M F (1987) Deficiency in DNA repair in mouse lymphoma strain L5178Y-S. Proc Natl Acad Sci U S A 84:7562-6 |
Evans, H H; Horng, M F; Beer, J Z (1986) Lethal and mutagenic effects of radiation and alkylating agents on two strains of mouse L5178Y cells. Mutat Res 161:91-7 |
Evans, H H; Mencl, J; Horng, M F et al. (1986) Locus specificity in the mutability of L5178Y mouse lymphoma cells: the role of multilocus lesions. Proc Natl Acad Sci U S A 83:4379-83 |