Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
3R01CA024432-31S1
Application #
3166427
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1986-09-01
Project End
1996-06-30
Budget Start
1993-07-01
Budget End
1994-06-30
Support Year
31
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199
Parhami-Seren, Behnaz; Krudysz, Jolanta; Tsantili, Panayota (2002) Affinity panning of peptide libraries using anti-streptokinase monoclonal antibodies: selection of an inhibitor of plasmin(ogen) active site. J Immunol Methods 267:185-98
Krykbaev, Rustem A; Tsantili, Panayota; Jeffrey, Philip D et al. (2002) Modifying specificity of antidigoxin antibodies using insertional mutagenesis. Protein Sci 11:2899-908
Parhami-Seren, Behnaz; Viswanathan, Malini; Margolies, Michael N (2002) Selection of high affinity p-azophenyarsonate Fabs from heavy-chain CDR2 insertion libraries. J Immunol Methods 259:43-53
Parhami-Seren, Behnaz; Haberly, Richard; Margolies, Michael N et al. (2002) Ouabain-binding protein(s) from human plasma. Hypertension 40:220-8
Parhami-Seren, B; Viswanathan, M; Strong, R K et al. (2001) Structural analysis of mutants of high-affinity and low-affinity p-azophenylarsonate-specific antibodies generated by alanine scanning of heavy chain complementarity-determining region 2. J Immunol 167:5129-35
Parhami-Seren, B; Bell, C; Margolies, M N et al. (1999) Monoclonal antibodies that distinguish between two related digitalis glycosides, ouabain and digoxin. J Immunol 163:4360-6
Wong, Y W; Gill, D S; Parhami-Seren, B et al. (1998) Structural requirements for a specificity switch and for maintenance of affinity using mutational analysis of a phage-displayed anti-arsonate antibody of Fab heavy chain first complementarity-determining region. J Immunol 160:5990-7
Gill, D S; Wong, Y W; Margolies, M N (1997) Differences in sequence-specific expression of two anti-arsonate Fabs in E. coli. Biotechnol Prog 13:692-4
Parhami-Seren, B; Keel, T; Reed, G L (1997) Sequences of antigenic epitopes of streptokinase identified via random peptide libraries displayed on phage. J Mol Biol 271:333-41
Parhami-Seren, B; Margolies, M N (1996) Contribution of heavy chain junctional amino acid diversity to antibody affinity among p-azophenylarsonate-specific antibodies. J Immunol 157:2066-72

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