Abstract

The major goal of this research program is to identify the gene products of Friend and Rauscher spleen focus forming viruses (SFFV), and to obtain evidence concerning their leukemogenic roles. One approach has been to clone these SFFVs into fibroblasts and to compare their gene products. Our work already accomplished suggests that these SFFVs are closely related to each other and to the dual tropic MuLVs which are env gene recombinants of ecotropic and xenotropic viruses. Although F-SFFV and R-SFFV have nearly identical recombinant env genes, they differ dramatically in their gag and pol genes. The R-SFFV genome encodes intact gag polyproteins and enzymatically active reverse transcriptase. We have obtained evidence that the gp55s encoded by SFFVs are heterogeneously metabolized due to a structural instability which results in efficient processing into the plasma membranes of infected cells. Recently we have succeeded in isolating two F-SFFV mutants with gp55 structural gene abnormalities, and we are currently analyzing the leukemogenic properties of these viral mutants.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA025810-08
Application #
3167021
Study Section
Virology Study Section (VR)
Project Start
1979-08-01
Project End
1987-07-31
Budget Start
1986-08-01
Budget End
1987-07-31
Support Year
8
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Oregon Health and Science University
Department
Type
Schools of Medicine
DUNS #
009584210
City
Portland
State
OR
Country
United States
Zip Code
97239

  Publications

Tailor, C S; Lavillette, D; Marin, M et al. (2003) Cell surface receptors for gammaretroviruses. Curr Top Microbiol Immunol 281:29-106
Marin, Mariana; Lavillette, Dimitri; Kelly, Sean M et al. (2003) N-linked glycosylation and sequence changes in a critical negative control region of the ASCT1 and ASCT2 neutral amino acid transporters determine their retroviral receptor functions. J Virol 77:2936-45
Lavillette, Dimitri; Marin, Mariana; Ruggieri, Alessia et al. (2002) The envelope glycoprotein of human endogenous retrovirus type W uses a divergent family of amino acid transporters/cell surface receptors. J Virol 76:6442-52
Tailor, C S; Marin, M; Nouri, A et al. (2001) Truncated forms of the dual function human ASCT2 neutral amino acid transporter/retroviral receptor are translationally initiated at multiple alternative CUG and GUG codons. J Biol Chem 276:27221-30
Tailor, C S; Nouri, A; Kabat, D (2000) Cellular and species resistance to murine amphotropic, gibbon ape, and feline subgroup C leukemia viruses is strongly influenced by receptor expression levels and by receptor masking mechanisms. J Virol 74:9797-801
Marin, M; Tailor, C S; Nouri, A et al. (2000) Sodium-dependent neutral amino acid transporter type 1 is an auxiliary receptor for baboon endogenous retrovirus. J Virol 74:8085-93
Tailor, C S; Nouri, A; Kabat, D (2000) A comprehensive approach to mapping the interacting surfaces of murine amphotropic and feline subgroup B leukemia viruses with their cell surface receptors. J Virol 74:237-44
Tailor, C S; Nouri, A; Zhao, Y et al. (1999) A sodium-dependent neutral-amino-acid transporter mediates infections of feline and baboon endogenous retroviruses and simian type D retroviruses. J Virol 73:4470-4
Tailor, C S; Willett, B J; Kabat, D (1999) A putative cell surface receptor for anemia-inducing feline leukemia virus subgroup C is a member of a transporter superfamily. J Virol 73:6500-5
Marin, M; Tailor, C S; Nouri, A et al. (1999) Polymorphisms of the cell surface receptor control mouse susceptibilities to xenotropic and polytropic leukemia viruses. J Virol 73:9362-8

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