Mason-Pfizer Monkey virus (M-PMV) is an exogenous, primate retrovirus that was isolated from a breast carcinoma of a rhesus monkey. It is the prototype virus of the D-type retroviruses which, like the B type retrovirus mouse mammary tumor virus, preassemble a complete capsid within the cytoplasm of a infected cell. These A-type particles migrate to the plasma membrane and are enveloped by a lipid bilayer upon release from the cell. The major goals of this proposal are to define those viral coded gag-gene functions that are essential in assembly, morphogenesis and infectivity of the M-PMV particle. The carboxy-terminal amino acid sequence of the five major gag gene products of this virus will be determined. This information will be used together with amino-terminal amino acid sequence information to define the polypeptide coding regions on the nucleic acid sequence of the gag gene determined from cloned M-PMV DNA. In vitro site directed mutagenesis of the gag gene coding region and of a sequence 3' of gag which may code for the viral protease will be used to determine the function of these regions. Deletion, insertion, and point mutagenesis procedures will be employed for these studies. The role of the envelope glycoproteins in directing the budding and release of viral particales will be investigated utilizing similar procedures.

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National Cancer Institute (NCI)
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Experimental Virology Study Section (EVR)
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University of Alabama Birmingham
School of Medicine & Dentistry
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Wen, Xiaoyun; Ding, Lingmei; Wang, Jaang-Jiun et al. (2014) ROCK1 and LIM kinase modulate retrovirus particle release and cell-cell transmission events. J Virol 88:6906-21
Wen, Xiaoyun; Ding, Lingmei; Hunter, Eric et al. (2014) An siRNA screen of membrane trafficking genes highlights pathways common to HIV-1 and M-PMV virus assembly and release. PLoS One 9:e106151
Pereira, Lara E; Clark, Jasmine; Grznarova, Petra et al. (2014) Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules. Virology 449:109-19
Clark, Jasmine; Grznarova, Petra; Stansell, Elizabeth et al. (2013) A Mason-Pfizer Monkey virus Gag-GFP fusion vector allows visualization of capsid transport in live cells and demonstrates a role for microtubules. PLoS One 8:e83863
Diehl, William E; Johnson, Welkin E; Hunter, Eric (2013) Elevated rate of fixation of endogenous retroviral elements in Haplorhini TRIM5 and TRIM22 genomic sequences: impact on transcriptional regulation. PLoS One 8:e58532
Prchal, Jan; Srb, Pavel; Hunter, Eric et al. (2012) The structure of myristoylated Mason-Pfizer monkey virus matrix protein and the role of phosphatidylinositol-(4,5)-bisphosphate in its membrane binding. J Mol Biol 423:427-38
Hadravova, Romana; de Marco, Alex; Ulbrich, Pavel et al. (2012) In vitro assembly of virus-like particles of a gammaretrovirus, the murine leukemia virus XMRV. J Virol 86:1297-306
Srb, Pavel; Vlach, Jiri; Prchal, Jan et al. (2011) Oligomerization of a retroviral matrix protein is facilitated by backbone flexibility on nanosecond time scale. J Phys Chem B 115:2634-44
Vorackova, Irena; Suchanova, Sarka; Ulbrich, Pavel et al. (2011) Purification of proteins containing zinc finger domains using immobilized metal ion affinity chromatography. Protein Expr Purif 79:88-95
Prchal, Jan; Junkova, Petra; Strmiskova, Miroslava et al. (2011) Expression and purification of myristoylated matrix protein of Mason-Pfizer monkey virus for NMR and MS measurements. Protein Expr Purif 79:122-7

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