Abstract

We propose a comparative study of transformation-specific genes and their products in the class of defective, rapidly transforming avian retroviruses. Our work will concentrate on several new virus isolates from spontaneous and chemically induced chicken tumors with the following specific aims: 1. Biological characterization of the new viruses will include tumor spectra, defectiveness of replication and properties of the transformed cells. 2. Nucleic acid studies will define transformation-related sequences with specific cDNA probes, RNase-T, fingerprints and heteroduplex mapping in the electron microscope. 3. Transformation-specific proteins will be characterized by two-dimensional tryptic peptide analysis, in vitro translation, tests for phosphokinase activity and reactivity with monoclonal antibodies. 4. Conditional and nonconditional mutants affecting the transforming ability of the new viruses will be sought. We expect that these studies will reveal common features of the transformation-related proteins in several groups of avian retroviruses and that the results will advance our knowledge of viral oncogenesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA029777-05
Application #
3168871
Study Section
Experimental Virology Study Section (EVR)
Project Start
1981-06-01
Project End
1986-05-31
Budget Start
1985-06-01
Budget End
1986-05-31
Support Year
5
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Southern California
Department
Type
Schools of Medicine
DUNS #
041544081
City
Los Angeles
State
CA
Country
United States
Zip Code
90033

  Publications

Vogt, P K (1987) Onc genes and signals of cell growth. Arzneimittelforschung 37:243-5
Vogt, P K; Bos, T J; Doolittle, R F (1987) Homology between the DNA-binding domain of the GCN4 regulatory protein of yeast and the carboxyl-terminal region of a protein coded for by the oncogene jun. Proc Natl Acad Sci U S A 84:3316-9
Benedict, S H (1987) Apparent DNA-binding protein specific for cells transformed by avian acute leukemia viruses. Cancer Res 47:6586-9
Maki, Y; Bos, T J; Davis, C et al. (1987) Avian sarcoma virus 17 carries the jun oncogene. Proc Natl Acad Sci U S A 84:2848-52
Woods, C M; Boyer, B; Vogt, P K et al. (1986) Control of erythroid differentiation: asynchronous expression of the anion transporter and the peripheral components of the membrane skeleton in AEV- and S13-transformed cells. J Cell Biol 103:1789-98
Nigg, E A; Sefton, B M; Singer, S J et al. (1986) Cytoskeletal organization, vinculin-phosphorylation, and fibronectin expression in transformed fibroblasts with different cell morphologies. Virology 151:50-65
Bos, T J; Beug, H; Graf, T et al. (1986) Two new retroviral onc genes, sea and jun. Princess Takamatsu Symp 17:23-30
Troesch, C D; Vogt, P K (1985) An endogenous virus from Lophortyx quail is the prototype for envelope subgroup 1 of avian retroviruses. Virology 143:595-602
Beug, H; Hayman, M J; Graf, T et al. (1985) S13, a rapidly oncogenic replication-defective avian retrovirus. Virology 145:141-53
Benedict, S H; Maki, Y; Vogt, P K (1985) Avian retrovirus S13: properties of the genome and of the transformation-specific protein. Virology 145:154-64

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